TY - JOUR
T1 - Xanthine Oxidase/Dehydrogenase is present in human placenta
AU - Many, A.
AU - Westerhausen-Larson, A.
AU - Kanbour-Shakir, A.
AU - Roberts, J. M.
PY - 1996
Y1 - 1996
N2 - Xanthine dehydrogenase/oxidase (XDH, EC 1.1.1.204, XO, EC 1.2.3.2) produces uric acid, and in the oxidase form also generates the free radical superoxide. Previous reports failed to demonstrate XDH/XO activity in human placenta. Our objective was to determine evidence of XDH/XO in human placenta. We developed a cDNA probe for human XDH/XO and used it to detect mRNA by Northern hybridization. Immunohistochemical localization of the enzyme in placental tissue was performed using a specific antibody for XDH/XO and ABC-peroxidase. Enzyme activity assay was determined by the conversion of [14C] xanthine to [14C] uric acid. was detected in all placental samples (n = 4). Villous and non-villous trophoblast cells expressed immunohistochemical for XOD (n = 4). Enzyme activity was detected in all placentae (n = 6). Despite previous reports, we found mRNA, XDH/XO protein and enzyme activity in human placenta localized to trophoblast cells. Enzyme activity was much lower than in liver. Several conditions in the maternal-fetal unit could potentially increase XDH/XO activity and conversion of the enzyme to its oxidase form.
AB - Xanthine dehydrogenase/oxidase (XDH, EC 1.1.1.204, XO, EC 1.2.3.2) produces uric acid, and in the oxidase form also generates the free radical superoxide. Previous reports failed to demonstrate XDH/XO activity in human placenta. Our objective was to determine evidence of XDH/XO in human placenta. We developed a cDNA probe for human XDH/XO and used it to detect mRNA by Northern hybridization. Immunohistochemical localization of the enzyme in placental tissue was performed using a specific antibody for XDH/XO and ABC-peroxidase. Enzyme activity assay was determined by the conversion of [14C] xanthine to [14C] uric acid. was detected in all placental samples (n = 4). Villous and non-villous trophoblast cells expressed immunohistochemical for XOD (n = 4). Enzyme activity was detected in all placentae (n = 6). Despite previous reports, we found mRNA, XDH/XO protein and enzyme activity in human placenta localized to trophoblast cells. Enzyme activity was much lower than in liver. Several conditions in the maternal-fetal unit could potentially increase XDH/XO activity and conversion of the enzyme to its oxidase form.
UR - http://www.scopus.com/inward/record.url?scp=0029884430&partnerID=8YFLogxK
U2 - 10.1016/S0143-4004(96)90061-2
DO - 10.1016/S0143-4004(96)90061-2
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C2 - 8829220
AN - SCOPUS:0029884430
SN - 0143-4004
VL - 17
SP - 361
EP - 365
JO - Placenta
JF - Placenta
IS - 5-6
ER -