TY - GEN
T1 - Wound healing assay of two competing cell types with dry mass measurement
AU - Cohen Maslaton, Shir
AU - Shaked, Natan T.
N1 - Publisher Copyright:
© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.
PY - 2019
Y1 - 2019
N2 - Wound healing assay is a method for evaluating of cell migration rate in vitro. In this assay, a scratch is performed in a confluent cell monolayer that usually contains only one type of cells. Interferometric phase microscopy (IPM) is a quantitative imaging technique, by which the optical path difference (OPD) of a sample is extracted. The OPD is equal to the thickness of the sample multiplied by its integral refractive index, and therefore provides us with quantitative information about the sample without labeling. Fluorescent probes are fluorescent compounds that can be attached to specific cells in order to provide ability of imaging the cells with specific contrast enhancement under fluorescence microscopy. Using a fluorescent microscope combined with an interferometric phase microscope, wound healing assay of a cell confluent monolayer that contains two types of cells is performed, while one type of cells is labeled with fluorescent probes for cell differentiation purposes. This allows us to check which cell type closes the wound faster, and to continually measure the dry mass of each cell population.
AB - Wound healing assay is a method for evaluating of cell migration rate in vitro. In this assay, a scratch is performed in a confluent cell monolayer that usually contains only one type of cells. Interferometric phase microscopy (IPM) is a quantitative imaging technique, by which the optical path difference (OPD) of a sample is extracted. The OPD is equal to the thickness of the sample multiplied by its integral refractive index, and therefore provides us with quantitative information about the sample without labeling. Fluorescent probes are fluorescent compounds that can be attached to specific cells in order to provide ability of imaging the cells with specific contrast enhancement under fluorescence microscopy. Using a fluorescent microscope combined with an interferometric phase microscope, wound healing assay of a cell confluent monolayer that contains two types of cells is performed, while one type of cells is labeled with fluorescent probes for cell differentiation purposes. This allows us to check which cell type closes the wound faster, and to continually measure the dry mass of each cell population.
KW - Cells migration
KW - Fluorescence microscopy
KW - Holography
KW - Quantitative phase imaging
KW - Wound healing
UR - http://www.scopus.com/inward/record.url?scp=85072630507&partnerID=8YFLogxK
U2 - 10.1117/12.2526841
DO - 10.1117/12.2526841
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AN - SCOPUS:85072630507
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV
A2 - Ferraro, Pietro
A2 - Grilli, Simonetta
A2 - Ritsch-Marte, Monika
A2 - Ritsch-Marte, Monika
A2 - Hitzenberger, Christoph K.
PB - SPIE
T2 - Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV 2019
Y2 - 24 June 2019 through 26 June 2019
ER -