Whole exome sequencing (WES) approach for diagnosing primary immunodeficiencies (PIDs) in a highly consanguineous community

Amos J. Simon, Adi Cohen Golan, Atar Lev, Tali Stauber, Ortal Barel, Ido Somekh, Christoph Klein, Omar AbuZaitun, Eran Eyal, Nitzan Kol, Ekrem Unal, Ninette Amariglio, Gideon Rechavi, Raz Somech*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Primary immunodeficiencies (PIDs) are a heterogeneous group of monogenic inborn errors of immunity. The genetic causes of these diseases can be identified using whole exome sequencing (WES). Here, DNA samples from 106 patients with a clinical suspicion of PID were subjected to WES in order to test the diagnostic yield of this test in a highly consanguineous community. A likely genetic diagnosis was achieved in 70% of patients. Several factors were considered to possibly influence the diagnostic rate of WES among our cohort including early age, presence of consanguinity, family history suggestive of PID, the number of family members who underwent WES and the clinical phenotype of the patient. The highest diagnostic rate was in patients with combined immunodeficiency or with a syndrome. Notably, WES findings altered the clinical management in 39% (41/106) of patients in our cohort. Our findings support the use of WES as an important diagnostic tool in patients with suspected PID, especially in highly consanguineous communities.

Original languageEnglish
Article number108376
JournalClinical Immunology
StatePublished - May 2020


FundersFunder number
Israel Ministry of Health
Israeli Science Foundation
Jeffrey Modell Foundation
Care-for-Rare Foundation
Israel Science Foundation
Ministry of Health, State of Israel
Sackler Faculty of Medicine, Tel-Aviv University


    • Autoimmunity
    • Bone marrow transplantation
    • Genetics
    • IVIG
    • Infections


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