Whole-exome sequencing in fetuses with central nervous system abnormalities

Adi Reches, Liran Hiersch, Sharon Simchoni, Dalit Barel, Rotem Greenberg, Liat Ben Sira, Gustavo Malinger, Yuval Yaron

Research output: Contribution to journalArticlepeer-review


Objective: We describe our experience with whole-exome sequencing (WES) in fetuses with central nervous system (CNS) abnormalities following a normal chromosomal microarray result. Methods: During the study period (2014–2017) 7 cases (9 fetuses) with prenatally diagnosed CNS abnormality, whose chromosomal microarray analysis was negative, were offered whole-exome sequencing analysis. Results: A pathogenic or a likely pathogenic variant was found in 5 cases including a previously described, likely pathogenic de novo TUBA1A variant (Case #1); a previously described homozygous VRK1 variant (Case #2); an X-linked ARX variant (Case #3); a likely pathogenic heterozygous variant in the TUBB3 gene (Case #5). Finally, in two fetuses of the same couple (Case #6), a compound heterozygous state was detected, consisting of the NPHP1 gene deletion and a sequence variant of uncertain significance. Two additional cases had normal WES results. Conclusion: Whole-exome sequencing may improve prenatal diagnosis in fetuses with CNS abnormalities.

Original languageEnglish
Pages (from-to)1301-1308
Number of pages8
JournalJournal of Perinatology
Issue number10
StatePublished - 1 Oct 2018


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