TY - JOUR
T1 - Whole-cell electrochemical biosensor integrating microbes with Si nanowire-forest
AU - Mintz-Hemed, Nofar
AU - Yoetz-Kopelman, Tal
AU - Convertino, Annalisa
AU - Freeman, Amihay
AU - Shacham-Diamand, Yosi
N1 - Publisher Copyright:
© 2017 The Electrochemical Society.
PY - 2017
Y1 - 2017
N2 - In the current work, the synergetic effect of microbial cells and functional nanostructures, such as highly disordered Si nanowires (SiNW-forest), has been exploited to develop a novel whole-cell biosensor. E.Coli microbial cells, expressing and displaying recombinant ZZ proteins, were immobilized onto SiNW-forest electrode. The existence of the microbial cells was verified visually by scanning electron microscopy, and viable counting, and electrochemically using impedance spectroscopy (EIS). The electrochemical impedance spectra show a clear dependence on the number of the microbial cells by allowing the detection of different number of cells in the range between 103 and 107. In addition, the EIS investigation suggests further decrease in the detected cells concentration to be possible.
AB - In the current work, the synergetic effect of microbial cells and functional nanostructures, such as highly disordered Si nanowires (SiNW-forest), has been exploited to develop a novel whole-cell biosensor. E.Coli microbial cells, expressing and displaying recombinant ZZ proteins, were immobilized onto SiNW-forest electrode. The existence of the microbial cells was verified visually by scanning electron microscopy, and viable counting, and electrochemically using impedance spectroscopy (EIS). The electrochemical impedance spectra show a clear dependence on the number of the microbial cells by allowing the detection of different number of cells in the range between 103 and 107. In addition, the EIS investigation suggests further decrease in the detected cells concentration to be possible.
UR - http://www.scopus.com/inward/record.url?scp=85035083329&partnerID=8YFLogxK
U2 - 10.1149/2.1471706jes
DO - 10.1149/2.1471706jes
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AN - SCOPUS:85035083329
SN - 0013-4651
VL - 164
SP - B253-B257
JO - Journal of the Electrochemical Society
JF - Journal of the Electrochemical Society
IS - 6
ER -