Whole-cell biochips for bio-sensing: Integration of live cells and inanimate surfaces

Hadar Ben-Yoav, Sahar Melamed, Amihay Freeman, Yosi Shacham-Diamand, Shimshon Belkin*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

43 Scopus citations


Recent advances in the convergence of the biological, chemical, physical, and engineering sciences have opened new avenues of research into the interfacing of diverse biological moieties with inanimate platforms. A main aspect of this field, the integration of live cells with micro-machined platforms for high throughput and bio-sensing applications, is the subject of the present review. These unique hybrid systems are configured in a manner that ensures positioning of the cells in designated patterns, and enables cellular viability maintenance, and monitoring of cellular functionality. Here we review both animate and inanimate surface properties and how they affect cellular attachment, describe relevant modifications of both types of surfaces, list technologies for platform engineering and for cell deposition in the desired configurations, and discuss the influence of various deposition and immobilization methods on the viability and performance of the immobilized cells.

Original languageEnglish
Pages (from-to)337-353
Number of pages17
JournalCritical Reviews in Biotechnology
Issue number4
StatePublished - Dec 2011


FundersFunder number
BMBF-MOST Cooperation in Water TechnologyWT0601
European Community FP6027900
US-Israel Binational Research and Development Fund
United States - Israel Binational Agricultural Research and Development FundUS-3864-06


    • Adherence
    • Bacteria
    • Biological cell deposition
    • Biosensors
    • Cell-substrate interface
    • Surface modification


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