VSV-ΔG-Spike Candidate Vaccine Induces Protective Immunity and Protects K18-hACE2 Mice against SARS-CoV-2 Variants

Yfat Yahalom-Ronen; Hadas Tamir; Sharon Melamed; Boaz Politi; Hagit Achdout; Noam Erez; Ofir Israeli; Inbar Cohen-Gihon; Lilach Chery Mimran; Moria Barlev-Gross; Michal Mandelboim; Irit Orr; Ester Feldmesser; Shay Weiss; Adi Beth-Din; Nir Paran; Tomer Israely

doi:10.3390/v15061364

VSV-ΔG-Spike Candidate Vaccine Induces Protective Immunity and Protects K18-hACE2 Mice against SARS-CoV-2 Variants

Yfat Yahalom-Ronen, Hadas Tamir, Sharon Melamed, Boaz Politi, Hagit Achdout, Noam Erez, Ofir Israeli, Inbar Cohen-Gihon, Lilach Chery Mimran, Moria Barlev-Gross, Michal Mandelboim, Irit Orr, Ester Feldmesser, Shay Weiss, Adi Beth-Din, Nir Paran^*, Tomer Israely^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Since the emergence of the original SARS-CoV-2, several variants were described, raising questions as to the ability of recently developed vaccine platforms to induce immunity and provide protection against these variants. Here, we utilized the K18-hACE2 mouse model to show that VSV-ΔG-spike vaccination provides protection against several SARS-CoV-2 variants: alpha, beta, gamma, and delta. We show an overall robust immune response, regardless of variant identity, leading to reduction in viral load in target organs, prevention of morbidity and mortality, as well as prevention of severe brain immune response, which follows infection with various variants. Additionally, we provide a comprehensive comparison of the brain transcriptomic profile in response to infection with different variants of SARS-CoV-2 and show how vaccination prevents these disease manifestations. Taken together, these results highlight the robust VSV-ΔG-spike protective response against diverse SARS-CoV-2 variants, as well as its promising potential against newly arising variants.

Original language	English
Article number	1364
Journal	Viruses
Volume	15
Issue number	6
DOIs	https://doi.org/10.3390/v15061364
State	Published - Jun 2023
Externally published	Yes

Keywords

K18-hACE2
SARS-CoV-2
VSV-ΔG-Spike
brain
vaccine
variants

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/v15061364

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Yahalom-Ronen, Y., Tamir, H., Melamed, S., Politi, B., Achdout, H., Erez, N., Israeli, O., Cohen-Gihon, I., Chery Mimran, L., Barlev-Gross, M., Mandelboim, M., Orr, I., Feldmesser, E., Weiss, S., Beth-Din, A., Paran, N., & Israely, T. (2023). VSV-ΔG-Spike Candidate Vaccine Induces Protective Immunity and Protects K18-hACE2 Mice against SARS-CoV-2 Variants. Viruses, 15(6), Article 1364. https://doi.org/10.3390/v15061364

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abstract = "Since the emergence of the original SARS-CoV-2, several variants were described, raising questions as to the ability of recently developed vaccine platforms to induce immunity and provide protection against these variants. Here, we utilized the K18-hACE2 mouse model to show that VSV-ΔG-spike vaccination provides protection against several SARS-CoV-2 variants: alpha, beta, gamma, and delta. We show an overall robust immune response, regardless of variant identity, leading to reduction in viral load in target organs, prevention of morbidity and mortality, as well as prevention of severe brain immune response, which follows infection with various variants. Additionally, we provide a comprehensive comparison of the brain transcriptomic profile in response to infection with different variants of SARS-CoV-2 and show how vaccination prevents these disease manifestations. Taken together, these results highlight the robust VSV-ΔG-spike protective response against diverse SARS-CoV-2 variants, as well as its promising potential against newly arising variants.",

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author = "Yfat Yahalom-Ronen and Hadas Tamir and Sharon Melamed and Boaz Politi and Hagit Achdout and Noam Erez and Ofir Israeli and Inbar Cohen-Gihon and {Chery Mimran}, Lilach and Moria Barlev-Gross and Michal Mandelboim and Irit Orr and Ester Feldmesser and Shay Weiss and Adi Beth-Din and Nir Paran and Tomer Israely",

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year = "2023",

month = jun,

doi = "10.3390/v15061364",

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Yahalom-Ronen, Y, Tamir, H, Melamed, S, Politi, B, Achdout, H, Erez, N, Israeli, O, Cohen-Gihon, I, Chery Mimran, L, Barlev-Gross, M, Mandelboim, M, Orr, I, Feldmesser, E, Weiss, S, Beth-Din, A, Paran, N & Israely, T 2023, 'VSV-ΔG-Spike Candidate Vaccine Induces Protective Immunity and Protects K18-hACE2 Mice against SARS-CoV-2 Variants', Viruses, vol. 15, no. 6, 1364. https://doi.org/10.3390/v15061364

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T1 - VSV-ΔG-Spike Candidate Vaccine Induces Protective Immunity and Protects K18-hACE2 Mice against SARS-CoV-2 Variants

AU - Yahalom-Ronen, Yfat

AU - Tamir, Hadas

AU - Melamed, Sharon

AU - Politi, Boaz

AU - Achdout, Hagit

AU - Erez, Noam

AU - Israeli, Ofir

AU - Cohen-Gihon, Inbar

AU - Chery Mimran, Lilach

AU - Barlev-Gross, Moria

AU - Mandelboim, Michal

AU - Orr, Irit

AU - Feldmesser, Ester

AU - Weiss, Shay

AU - Beth-Din, Adi

AU - Paran, Nir

AU - Israely, Tomer

PY - 2023/6

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N2 - Since the emergence of the original SARS-CoV-2, several variants were described, raising questions as to the ability of recently developed vaccine platforms to induce immunity and provide protection against these variants. Here, we utilized the K18-hACE2 mouse model to show that VSV-ΔG-spike vaccination provides protection against several SARS-CoV-2 variants: alpha, beta, gamma, and delta. We show an overall robust immune response, regardless of variant identity, leading to reduction in viral load in target organs, prevention of morbidity and mortality, as well as prevention of severe brain immune response, which follows infection with various variants. Additionally, we provide a comprehensive comparison of the brain transcriptomic profile in response to infection with different variants of SARS-CoV-2 and show how vaccination prevents these disease manifestations. Taken together, these results highlight the robust VSV-ΔG-spike protective response against diverse SARS-CoV-2 variants, as well as its promising potential against newly arising variants.

AB - Since the emergence of the original SARS-CoV-2, several variants were described, raising questions as to the ability of recently developed vaccine platforms to induce immunity and provide protection against these variants. Here, we utilized the K18-hACE2 mouse model to show that VSV-ΔG-spike vaccination provides protection against several SARS-CoV-2 variants: alpha, beta, gamma, and delta. We show an overall robust immune response, regardless of variant identity, leading to reduction in viral load in target organs, prevention of morbidity and mortality, as well as prevention of severe brain immune response, which follows infection with various variants. Additionally, we provide a comprehensive comparison of the brain transcriptomic profile in response to infection with different variants of SARS-CoV-2 and show how vaccination prevents these disease manifestations. Taken together, these results highlight the robust VSV-ΔG-spike protective response against diverse SARS-CoV-2 variants, as well as its promising potential against newly arising variants.

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VSV-ΔG-Spike Candidate Vaccine Induces Protective Immunity and Protects K18-hACE2 Mice against SARS-CoV-2 Variants

Abstract

Keywords

UN SDGs

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