Vliianie fragmenta DNK plazmidy pBR322 v oblasti gena ustoǐchivosti k tetratsiklinu na stabil'nost' étoǐ plazmidy.

M. N. Kolot; M. V. Kashlev; A. I. Gragerov; I. A. Khmel'

Vliianie fragmenta DNK plazmidy pBR322 v oblasti gena ustoǐchivosti k tetratsiklinu na stabil'nost' étoǐ plazmidy.

Translated title of the contribution: Effect of a fragment of pBR322 plasmid in the region of tetracycline resistance gene on the stability of this plasmid

M. N. Kolot^*, M. V. Kashlev, A. I. Gragerov, I. A. Khmel'

^*Corresponding author for this work

Biochemistry Molecular Biology

Research output: Contribution to journal › Article › peer-review

Abstract

A fragment destabilizing the pBR322 plasmid has been localized in the region of pBR322 tet gene promoter. On the basis of stability analysis of pBR322 derivatives comprising the modified region of tet gene, we deduced that it is the DNA sequence localized at the beginning of tet gene in the region of HindIII splitting site that ensures the plasmid destabilization, and not tet gene product or the active transcription of this region. The destabilizing effect of this fragment of the plasmid is manifested both in cis and in trans. Possible molecular mechanisms of the phenomenon are discussed.

Translated title of the contribution	Effect of a fragment of pBR322 plasmid in the region of tetracycline resistance gene on the stability of this plasmid
Original language	Russian
Pages (from-to)	1301-1306
Number of pages	6
Journal	Molekulyarnaya Biologiya
Volume	22
Issue number	5
State	Published - Sep 1988

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title = "Vliianie fragmenta DNK plazmidy pBR322 v oblasti gena ustoǐchivosti k tetratsiklinu na stabil'nost' {\'e}toǐ plazmidy.",

abstract = "A fragment destabilizing the pBR322 plasmid has been localized in the region of pBR322 tet gene promoter. On the basis of stability analysis of pBR322 derivatives comprising the modified region of tet gene, we deduced that it is the DNA sequence localized at the beginning of tet gene in the region of HindIII splitting site that ensures the plasmid destabilization, and not tet gene product or the active transcription of this region. The destabilizing effect of this fragment of the plasmid is manifested both in cis and in trans. Possible molecular mechanisms of the phenomenon are discussed.",

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AU - Kolot, M. N.

AU - Kashlev, M. V.

AU - Gragerov, A. I.

AU - Khmel', I. A.

PY - 1988/9

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N2 - A fragment destabilizing the pBR322 plasmid has been localized in the region of pBR322 tet gene promoter. On the basis of stability analysis of pBR322 derivatives comprising the modified region of tet gene, we deduced that it is the DNA sequence localized at the beginning of tet gene in the region of HindIII splitting site that ensures the plasmid destabilization, and not tet gene product or the active transcription of this region. The destabilizing effect of this fragment of the plasmid is manifested both in cis and in trans. Possible molecular mechanisms of the phenomenon are discussed.

AB - A fragment destabilizing the pBR322 plasmid has been localized in the region of pBR322 tet gene promoter. On the basis of stability analysis of pBR322 derivatives comprising the modified region of tet gene, we deduced that it is the DNA sequence localized at the beginning of tet gene in the region of HindIII splitting site that ensures the plasmid destabilization, and not tet gene product or the active transcription of this region. The destabilizing effect of this fragment of the plasmid is manifested both in cis and in trans. Possible molecular mechanisms of the phenomenon are discussed.

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Vliianie fragmenta DNK plazmidy pBR322 v oblasti gena ustoǐchivosti k tetratsiklinu na stabil'nost' étoǐ plazmidy.

Abstract

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