TY - JOUR
T1 - Virulence variation of cucurbit powdery mildews in the Czech Republic – population approach
AU - Lebeda, Aleš
AU - Křístková, Eva
AU - Sedláková, Božena
AU - McCreight, James D.
AU - Kosman, Evsey
N1 - Publisher Copyright:
© 2018, Koninklijke Nederlandse Planteziektenkundige Vereniging.
PY - 2018/10/1
Y1 - 2018/10/1
N2 - Kosman diversity models were applied to analyses of virulence (disease reaction patterns) variation of 115 isolates of two cucurbit powdery mildew (CPM) species, Golovinomyces orontii (Go) and Podosphaera xanthii (Px), collected in the Czech Republic from 2010 through 2012. Diversity within and distances between Go and Px populations and each other in a spatio-temporal context and with regard to original host plant species were analyzed based on virulence patterns of individual isolates on a set of 21 melon (Cucumis melo L.) race differential genotypes. Significant differentiation among the Go and Px pathogen populations was revealed, and the results clearly demonstrate and confirm that the set of differential C. melo genotypes is well composed because of high differentiation capacity. Differentiation of pathogens among years was significant for both species. No significant difference between Go isolates from different host plant species was established due to high variability among Go isolates, but there was significant host-specific differentiation among Px isolates. Differentiation of pathogens among regions was not detected. These results revealed high virulence variation in isolates of Go and Px, and their spatio-temporal fluctuations. High diversity in virulence of Go isolates supports the treatment of Go as a complex of different (sub)species with distinct virulence factors. Similar relationships of selected Go isolates in a UPGMA dendrogram in a previously reported multigene phylogenetic tree support the logic and suitability of the Kosman assignment based approach to population studies of organisms with asexual or mixed modes of reproduction. The approach applied in this study provides a complex view of virulence structures of powdery mildew populations, and when combined with race determination and denomination on melon, it may serve as a base to understandvirulence variation of these CPM species from a spatio-temporal viewpoint.
AB - Kosman diversity models were applied to analyses of virulence (disease reaction patterns) variation of 115 isolates of two cucurbit powdery mildew (CPM) species, Golovinomyces orontii (Go) and Podosphaera xanthii (Px), collected in the Czech Republic from 2010 through 2012. Diversity within and distances between Go and Px populations and each other in a spatio-temporal context and with regard to original host plant species were analyzed based on virulence patterns of individual isolates on a set of 21 melon (Cucumis melo L.) race differential genotypes. Significant differentiation among the Go and Px pathogen populations was revealed, and the results clearly demonstrate and confirm that the set of differential C. melo genotypes is well composed because of high differentiation capacity. Differentiation of pathogens among years was significant for both species. No significant difference between Go isolates from different host plant species was established due to high variability among Go isolates, but there was significant host-specific differentiation among Px isolates. Differentiation of pathogens among regions was not detected. These results revealed high virulence variation in isolates of Go and Px, and their spatio-temporal fluctuations. High diversity in virulence of Go isolates supports the treatment of Go as a complex of different (sub)species with distinct virulence factors. Similar relationships of selected Go isolates in a UPGMA dendrogram in a previously reported multigene phylogenetic tree support the logic and suitability of the Kosman assignment based approach to population studies of organisms with asexual or mixed modes of reproduction. The approach applied in this study provides a complex view of virulence structures of powdery mildew populations, and when combined with race determination and denomination on melon, it may serve as a base to understandvirulence variation of these CPM species from a spatio-temporal viewpoint.
KW - Asexual (clonal) reproduction
KW - Assignment based approach
KW - Cucumis melo
KW - Differential set validation
KW - Golovinomyces orontii
KW - Podosphaera xanthii
KW - Population diversity
KW - Virulence analysis tool (VAT) software
KW - Virulence patterns
UR - http://www.scopus.com/inward/record.url?scp=85044444632&partnerID=8YFLogxK
U2 - 10.1007/s10658-018-1476-x
DO - 10.1007/s10658-018-1476-x
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AN - SCOPUS:85044444632
SN - 0929-1873
VL - 152
SP - 309
EP - 326
JO - European Journal of Plant Pathology
JF - European Journal of Plant Pathology
IS - 2
ER -