TY - JOUR
T1 - Virulence patterns from septicemic Escherichia coli O78 strains
AU - Babai, Reuven
AU - Blum-Oehler, Gabriele
AU - Stern, Baruch E.
AU - Hacker, Jörg
AU - Ron, Eliora Z.
PY - 1997/4/1
Y1 - 1997/4/1
N2 - Several septicemic Escherichia coli O78 strains, isolated from different sources, were characterized phenotypically and genotypically. Two avian isolates, one of which is known to carry the AC/I fimbriae, hybridized with the sfa determinant in colony dot-blot assay. Southern hybridizations with specific sfa probes, following pulsed-field gel electrophoresis (PFGE), showed positive hybridization to the same fragment in each of these strains. Determination of the N-terminal amino acid sequence of the AC/I major subunit gene revealed high similarity to the sequence of the SfaA-II protein. These data suggest that the adhesin gene cluster, coding for AC/I fimbriae, belongs to the S-fimbrial adhesin family.
AB - Several septicemic Escherichia coli O78 strains, isolated from different sources, were characterized phenotypically and genotypically. Two avian isolates, one of which is known to carry the AC/I fimbriae, hybridized with the sfa determinant in colony dot-blot assay. Southern hybridizations with specific sfa probes, following pulsed-field gel electrophoresis (PFGE), showed positive hybridization to the same fragment in each of these strains. Determination of the N-terminal amino acid sequence of the AC/I major subunit gene revealed high similarity to the sequence of the SfaA-II protein. These data suggest that the adhesin gene cluster, coding for AC/I fimbriae, belongs to the S-fimbrial adhesin family.
KW - AC/I fimbria
KW - Escherichia coli serotype O78
KW - S-Fimbria
KW - Septicemia
KW - Virulence factor
UR - http://www.scopus.com/inward/record.url?scp=0030966130&partnerID=8YFLogxK
U2 - 10.1016/S0378-1097(97)00062-1
DO - 10.1016/S0378-1097(97)00062-1
M3 - מאמר
AN - SCOPUS:0030966130
VL - 149
SP - 99
EP - 105
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 1
ER -