TY - JOUR
T1 - VEGF-induced adult neovascularization
T2 - Recruitment, retention, and role of accessory cells
AU - Grunewald, Myriam
AU - Avraham, Inbal
AU - Dor, Yuval
AU - Bachar-Lustig, Esther
AU - Itin, Ahuva
AU - Yung, Steffen
AU - Chimenti, Stephano
AU - Landsman, Limor
AU - Abramovitch, Rinat
AU - Keshet, Eli
N1 - Funding Information:
We thank Drs. Amnon Peled, Tamar Licht, Anat Globerman, and Dalit May for their valuable contributions and the Israel Science Foundation and the European Vascular Genomic Network (EVGN) for financial support. M.G. was supported by a postdoctoral fellowship from the Israel Cancer Research Fund (ICRF) and from the Ministry of Science, Israel.
PY - 2006/1/13
Y1 - 2006/1/13
N2 - Adult neovascularization relies on the recruitment of circulating cells, but their angiogenic roles and recruitment mechanisms are unclear. We show that the endothelial growth factor VEGF is sufficient for organ homing of circulating mononuclear myeloid cells and is required for their perivascular positioning and retention. Recruited bone marrow-derived circulating cells (RBCCs) summoned by VEGF serve a function distinct from endothelial progenitor cells. Retention of RBCCs in close proximity to angiogenic vessels is mediated by SDF1, a chemokine induced by VEGF in activated perivascular myofibroblasts. RBCCs enhance in situ proliferation of endothelial cells via secreting proangiogenic activities distinct from locally induced activities. Precluding RBCCs strongly attenuated the proangiogenic response to VEGF and addition of purified RBCCs enhanced angiogenesis in excision wounds. Together, the data suggest a model for VEGF-programmed adult neovascularization highlighting the essential paracrine role of recruited myeloid cells and a role for SDF1 in their perivascular retention.
AB - Adult neovascularization relies on the recruitment of circulating cells, but their angiogenic roles and recruitment mechanisms are unclear. We show that the endothelial growth factor VEGF is sufficient for organ homing of circulating mononuclear myeloid cells and is required for their perivascular positioning and retention. Recruited bone marrow-derived circulating cells (RBCCs) summoned by VEGF serve a function distinct from endothelial progenitor cells. Retention of RBCCs in close proximity to angiogenic vessels is mediated by SDF1, a chemokine induced by VEGF in activated perivascular myofibroblasts. RBCCs enhance in situ proliferation of endothelial cells via secreting proangiogenic activities distinct from locally induced activities. Precluding RBCCs strongly attenuated the proangiogenic response to VEGF and addition of purified RBCCs enhanced angiogenesis in excision wounds. Together, the data suggest a model for VEGF-programmed adult neovascularization highlighting the essential paracrine role of recruited myeloid cells and a role for SDF1 in their perivascular retention.
UR - http://www.scopus.com/inward/record.url?scp=30344437303&partnerID=8YFLogxK
U2 - 10.1016/j.cell.2005.10.036
DO - 10.1016/j.cell.2005.10.036
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C2 - 16413490
AN - SCOPUS:30344437303
SN - 0092-8674
VL - 124
SP - 175
EP - 189
JO - Cell
JF - Cell
IS - 1
ER -