TY - JOUR
T1 - Vasoactive intestinal peptide regulates embryonic growth through the action of activity-dependent neurotrophic factor
AU - Hill, Joanna M.
AU - Glazner, Gordon W.
AU - Susan J, Lee
AU - Gozes, Illana
AU - Gressens, Pierre
AU - Brenneman, Douglas E.
PY - 1999
Y1 - 1999
N2 - Activity-dependent neurotrophic factor is a potent, neuroprotective protein released from astroglia by VIP and accounts in part for the neuro protective properties of this neuropeptide. The growth-regulatory actions of VIP during embryogenesis may also occur indirectly through the release of activity-dependent neurotrophic factor. Whole cultured day-9 mouse embryos treated with activity-dependent neurotrophic factor (10-13 M) for 4 hr grew 3.1 somites, compared with 1.6 somites in control embryos. Treated embryos appeared morphologically normal and exhibited significant increases in cross-sectional area, protein, and DNA content and bromodeoxyuridine incorporation. Anti-activity-dependent neurotrophic factor significantly inhibited growth. Co-treatment of embryos with anti-activity-dependent neurotrophic factor inhibited VIP-stimulated growth; however, anti-VIP did not inhibit activity-dependent neurotrophic: factor-induced growth. These data indicate that an activity-dependent neurotrophic factor-like substance is an endogenous embryonic growth factor and that VIP-regulated growth occurs, at least in part, through activity-dependent neurotrophic factor.
AB - Activity-dependent neurotrophic factor is a potent, neuroprotective protein released from astroglia by VIP and accounts in part for the neuro protective properties of this neuropeptide. The growth-regulatory actions of VIP during embryogenesis may also occur indirectly through the release of activity-dependent neurotrophic factor. Whole cultured day-9 mouse embryos treated with activity-dependent neurotrophic factor (10-13 M) for 4 hr grew 3.1 somites, compared with 1.6 somites in control embryos. Treated embryos appeared morphologically normal and exhibited significant increases in cross-sectional area, protein, and DNA content and bromodeoxyuridine incorporation. Anti-activity-dependent neurotrophic factor significantly inhibited growth. Co-treatment of embryos with anti-activity-dependent neurotrophic factor inhibited VIP-stimulated growth; however, anti-VIP did not inhibit activity-dependent neurotrophic: factor-induced growth. These data indicate that an activity-dependent neurotrophic factor-like substance is an endogenous embryonic growth factor and that VIP-regulated growth occurs, at least in part, through activity-dependent neurotrophic factor.
UR - http://www.scopus.com/inward/record.url?scp=0033377850&partnerID=8YFLogxK
U2 - 10.1111/j.1749-6632.1999.tb07881.x
DO - 10.1111/j.1749-6632.1999.tb07881.x
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C2 - 10676438
AN - SCOPUS:0033377850
SN - 0077-8923
VL - 897
SP - 92
EP - 100
JO - Annals of the New York Academy of Sciences
JF - Annals of the New York Academy of Sciences
ER -