Vasoactive intestinal peptide regulates embryonic growth through the action of activity-dependent neurotrophic factor

Joanna M. Hill*, Gordon W. Glazner, Lee Susan J, Illana Gozes, Pierre Gressens, Douglas E. Brenneman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Activity-dependent neurotrophic factor is a potent, neuroprotective protein released from astroglia by VIP and accounts in part for the neuro protective properties of this neuropeptide. The growth-regulatory actions of VIP during embryogenesis may also occur indirectly through the release of activity-dependent neurotrophic factor. Whole cultured day-9 mouse embryos treated with activity-dependent neurotrophic factor (10-13 M) for 4 hr grew 3.1 somites, compared with 1.6 somites in control embryos. Treated embryos appeared morphologically normal and exhibited significant increases in cross-sectional area, protein, and DNA content and bromodeoxyuridine incorporation. Anti-activity-dependent neurotrophic factor significantly inhibited growth. Co-treatment of embryos with anti-activity-dependent neurotrophic factor inhibited VIP-stimulated growth; however, anti-VIP did not inhibit activity-dependent neurotrophic: factor-induced growth. These data indicate that an activity-dependent neurotrophic factor-like substance is an endogenous embryonic growth factor and that VIP-regulated growth occurs, at least in part, through activity-dependent neurotrophic factor.

Original languageEnglish
Pages (from-to)92-100
Number of pages9
JournalAnnals of the New York Academy of Sciences
Volume897
DOIs
StatePublished - 1999

Funding

FundersFunder number
Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentZ01HD000047

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