Vasoactive Intestinal Peptide Gene: Putative Mechanism of Information Storage at the RNA Level

Illana Gozes*, Eliezer Giladi, Yael Shani

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract: The human gene coding for vasoactive intestinal peptide was recently isolated and shown to contain seven exons. We now demonstrate that an intron‐containing precursor RNA can be the major vasoactive intestinal peptide‐related RNA in the cell, which is in contrast to most known genes. By RNA blot analysis using a variety of genomic and cDNA‐related probes we show that in a human tumor producing vasoactive intestinal peptide, most of the RNA encoding the peptide is of the precursor type. Similar precursor transcripts were found in total rat brain RNA as well. As proof of the identity of the intron‐containing RNA, cDNA clones corresponding to this RNA sequence have been isolated.

Original languageEnglish
Pages (from-to)1136-1141
Number of pages6
JournalJournal of Neurochemistry
Volume48
Issue number4
DOIs
StatePublished - Apr 1987
Externally publishedYes

Keywords

  • RNA blot hybridization)
  • Vasoactive intestinal peptide gene
  • Vasoactive intestinal peptide precursor RNA
  • cDNA cloning

Fingerprint

Dive into the research topics of 'Vasoactive Intestinal Peptide Gene: Putative Mechanism of Information Storage at the RNA Level'. Together they form a unique fingerprint.

Cite this