Vasoactive intestinal peptide and related molecules induce nitrite accumulation in the extracellular milieu of rat cerebral cortical cultures

Osnat Ashur-Fabian, Eliezer Giladi, Sharon Furman, Ruth A. Steingart, Yoram Wollman, Mati Fridkin, Douglas E. Brenneman, Illana Gozes*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Nanomolar concentrations of vasoactive intestinal peptide (VIP), picomolar concentrations of stearyl-norleucine 17-VIP (SNV) and femtomolar concentrations of NAPVSIPQ (NAP), an 8-amino-acid peptide derived from the VIP-responsive activity-dependent neuroprotective protein, provide broad neuroprotection. In rat cerebral cortical cultures, 10 -16-10 -7 M NAP increased intracellular cyclic guanosine monophosphate (cGMP) (2.5-4-fold) and 10 -10 M NAP increased extracellular nitric oxide (NO) by 60%. In the same culture system, VIP and SNV (at micromolar concentrations) increased extracellular NO by 45-55%. The NAP dose required for cGMP increases correlated with the dose providing neuroprotection. However, the concentrations of NAP, SNV and VIP affecting NO production did not match the neuro-protective doses. Thus, NO may mediate part of the cell-cell interaction and natural maintenance activity of VIP/SNV/NAP, while cGMP may mediate neuroprotection.

Original languageEnglish
Pages (from-to)167-170
Number of pages4
JournalNeuroscience Letters
Volume307
Issue number3
DOIs
StatePublished - 20 Jul 2001

Keywords

  • Activity-dependent neuroprotective protein
  • Cortical cultures
  • Cyclic guanosine monophosphate
  • Nitric Oxide
  • Stearyl-norleucine 17-Vasoactive intestinal peptide
  • Vasoactive intestinal peptide

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