Variable electric fields for high throughput electroporation protocol design in curvilinear coordinates

Francois Fernand; Liel Rubinsky; Alex Golberg; Boris Rubinsky

doi:10.1002/bit.24479

Variable electric fields for high throughput electroporation protocol design in curvilinear coordinates

Francois Fernand
, Liel Rubinsky
, Alex Golberg^*
, Boris Rubinsky

^*Corresponding author for this work

University of California at Berkeley

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The mathematical solution to the electric field equation in cylindrical coordinates, has suggested to us a new experimental methodology and device for reducing experimental effort in designing electroporation protocols. Using a new cylindrical electroporation system, we show, with an Escherichia coli cell model, how key electroporation parameters emerge precisely from single experiments rather than through interpolation from numerous experiments in the conventional Cartesian electroporation system.

Original language	English
Pages (from-to)	2168-2171
Number of pages	4
Journal	Biotechnology and Bioengineering
Volume	109
Issue number	8
DOIs	https://doi.org/10.1002/bit.24479
State	Published - Aug 2012
Externally published	Yes

Keywords

Electroporation
High throughput
Protocol design

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10.1002/bit.24479

Cite this

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title = "Variable electric fields for high throughput electroporation protocol design in curvilinear coordinates",

abstract = "The mathematical solution to the electric field equation in cylindrical coordinates, has suggested to us a new experimental methodology and device for reducing experimental effort in designing electroporation protocols. Using a new cylindrical electroporation system, we show, with an Escherichia coli cell model, how key electroporation parameters emerge precisely from single experiments rather than through interpolation from numerous experiments in the conventional Cartesian electroporation system.",

keywords = "Electroporation, High throughput, Protocol design",

author = "Francois Fernand and Liel Rubinsky and Alex Golberg and Boris Rubinsky",

year = "2012",

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AU - Fernand, Francois

AU - Rubinsky, Liel

AU - Golberg, Alex

AU - Rubinsky, Boris

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AB - The mathematical solution to the electric field equation in cylindrical coordinates, has suggested to us a new experimental methodology and device for reducing experimental effort in designing electroporation protocols. Using a new cylindrical electroporation system, we show, with an Escherichia coli cell model, how key electroporation parameters emerge precisely from single experiments rather than through interpolation from numerous experiments in the conventional Cartesian electroporation system.

KW - Electroporation

KW - High throughput

KW - Protocol design

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Variable electric fields for high throughput electroporation protocol design in curvilinear coordinates

Abstract

Keywords

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