Utilization of murine laparoscopy for continuous in-vivo assessment of the liver in multiple disease models

Yami Shapira*, Meirav Katz, Muhammad Ali, Michael Kaplan, Eli Brazowski, Zamir Halpern, Eran Elinav

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Background: Current strategies for follow up of murine models of liver disease are flawed by inability to continuously monitor disease progression in the tissue level, and necessitate sacrifice of animals for tissue sampling. Aims: In this study we aimed at developing a safe repetitive tool for sampling livers in vivo, by utilization of a miniaturized endoscopy system for laparoscopic liver biopsies and for injection of tumor cells into livers. Results: We report the development of a protocol for murine laparoscopy that allows repeated visualization of murine intraabdominal organs. The system enables safe and repeated liver biopsies in mice and rats, yielding adequate tissue for histological staining and RNA extraction. In addition, injection of tumor cells into livers facilitates under-vision implantation of hepatic tumors in liver, followed by visualization of tumor growth. Conclusions: Murine laparoscopy may be employed as a novel imaging modality for continuous assessment and manipulation of chronic liver disease models.

Original languageEnglish
Article numbere4776
JournalPLoS ONE
Volume4
Issue number3
DOIs
StatePublished - 10 Mar 2009
Externally publishedYes

Fingerprint

Dive into the research topics of 'Utilization of murine laparoscopy for continuous in-vivo assessment of the liver in multiple disease models'. Together they form a unique fingerprint.

Cite this