Use of soybean agglutinin for the separation of mouse B and T lymphocytes

Yair Reisner*, Amiram Ravid, Nathan Sharon

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

90 Scopus citations

Abstract

A new approach for the separation of lymphocyte subpopulations based on differential agglutinability with lectins is described. Mouse spleen B cells were agglutinated by soybean agglutinin while the T cells were not. The B cell aggregates were separated by gravity sedimentation and were dissociated into viable single cells by the addition of d-galactose, a hapten inhibitor of soybean agglutinin. The two cell fractions were characterized by their surface antigens, by their graft versus host activity and by their response to various mitogens.

Original languageEnglish
Pages (from-to)1585-1591
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume72
Issue number4
DOIs
StatePublished - 18 Oct 1976
Externally publishedYes

Funding

FundersFunder number
Weizmann Institute in Buenos Aires
United States-Israel Binational Science Foundation

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