TY - JOUR
T1 - Use of soybean agglutinin for the separation of mouse B and T lymphocytes
AU - Reisner, Yair
AU - Ravid, Amiram
AU - Sharon, Nathan
N1 - Funding Information:
This work was supported in part by a grant from the United States-Israel Binational Science Foundation, and by a contribution from a friend of the Weizmann Institute in Buenos Aires, Argentina. N.S. is an Established Investigator of the Chief Scientist's Bureau, Israel Ministry of Health.
PY - 1976/10/18
Y1 - 1976/10/18
N2 - A new approach for the separation of lymphocyte subpopulations based on differential agglutinability with lectins is described. Mouse spleen B cells were agglutinated by soybean agglutinin while the T cells were not. The B cell aggregates were separated by gravity sedimentation and were dissociated into viable single cells by the addition of d-galactose, a hapten inhibitor of soybean agglutinin. The two cell fractions were characterized by their surface antigens, by their graft versus host activity and by their response to various mitogens.
AB - A new approach for the separation of lymphocyte subpopulations based on differential agglutinability with lectins is described. Mouse spleen B cells were agglutinated by soybean agglutinin while the T cells were not. The B cell aggregates were separated by gravity sedimentation and were dissociated into viable single cells by the addition of d-galactose, a hapten inhibitor of soybean agglutinin. The two cell fractions were characterized by their surface antigens, by their graft versus host activity and by their response to various mitogens.
UR - http://www.scopus.com/inward/record.url?scp=0017133144&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(76)80195-7
DO - 10.1016/S0006-291X(76)80195-7
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AN - SCOPUS:0017133144
SN - 0006-291X
VL - 72
SP - 1585
EP - 1591
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -