A new approach for the separation of lymphocyte subpopulations based on differential agglutinability with lectins is described. Mouse spleen B cells were agglutinated by soybean agglutinin while the T cells were not. The B cell aggregates were separated by gravity sedimentation and were dissociated into viable single cells by the addition of d-galactose, a hapten inhibitor of soybean agglutinin. The two cell fractions were characterized by their surface antigens, by their graft versus host activity and by their response to various mitogens.
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - 18 Oct 1976|