Use of protein-bacteriophage conjugates for detection and quantitation of proteins

Joseph Haimovich*, Esther Hurwitz, Noa Novik, Michael Sela

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The immunospecific inactivation of protein-bacteriophage conjugates with anti-protein antibodies can be inhibited by free protein, identical or similar to that coupled to the bacteriophage. This serves as an assay for the sensitive detection and quantitation of proteins. The threshold concentrations detectable were, for example, 0.4 ng/ml for lysozyme and 0.05 ng/ml for rabbit immunoglobulin G. As little as 7 μunits/ml of insulin could be detected by this method in samples of human sera. The concentration of insulin determined by the inhibition of inactivation of insulin-bacteriophage T4 conjugate compared well, in most instances, with the results obtained by radio-immunoassay.

Original languageEnglish
Pages (from-to)125-129
Number of pages5
JournalBBA - Protein Structure
Issue number1
StatePublished - 28 Apr 1970
Externally publishedYes


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