TY - JOUR
T1 - Use of protein-bacteriophage conjugates for detection and quantitation of proteins
AU - Haimovich, Joseph
AU - Hurwitz, Esther
AU - Novik, Noa
AU - Sela, Michael
PY - 1970/4/28
Y1 - 1970/4/28
N2 - The immunospecific inactivation of protein-bacteriophage conjugates with anti-protein antibodies can be inhibited by free protein, identical or similar to that coupled to the bacteriophage. This serves as an assay for the sensitive detection and quantitation of proteins. The threshold concentrations detectable were, for example, 0.4 ng/ml for lysozyme and 0.05 ng/ml for rabbit immunoglobulin G. As little as 7 μunits/ml of insulin could be detected by this method in samples of human sera. The concentration of insulin determined by the inhibition of inactivation of insulin-bacteriophage T4 conjugate compared well, in most instances, with the results obtained by radio-immunoassay.
AB - The immunospecific inactivation of protein-bacteriophage conjugates with anti-protein antibodies can be inhibited by free protein, identical or similar to that coupled to the bacteriophage. This serves as an assay for the sensitive detection and quantitation of proteins. The threshold concentrations detectable were, for example, 0.4 ng/ml for lysozyme and 0.05 ng/ml for rabbit immunoglobulin G. As little as 7 μunits/ml of insulin could be detected by this method in samples of human sera. The concentration of insulin determined by the inhibition of inactivation of insulin-bacteriophage T4 conjugate compared well, in most instances, with the results obtained by radio-immunoassay.
UR - http://www.scopus.com/inward/record.url?scp=0014965902&partnerID=8YFLogxK
U2 - 10.1016/0005-2795(70)90142-X
DO - 10.1016/0005-2795(70)90142-X
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AN - SCOPUS:0014965902
SN - 0005-2795
VL - 207
SP - 125
EP - 129
JO - BBA - Protein Structure
JF - BBA - Protein Structure
IS - 1
ER -