Use of Antibodies against the Human Antigen of Erythroblasts for the Detection of Nucleated Erythrocytes in the Maternal Circulation

Shoshana Savion; Howard Carp; Jeanne Shepshelovich; Joseph Irlin; Michael Kostykov; Amos Fein; Vladimir Toder

doi:10.1159/000244406

Use of Antibodies against the Human Antigen of Erythroblasts for the Detection of Nucleated Erythrocytes in the Maternal Circulation

Shoshana Savion^*, Howard Carp, Jeanne Shepshelovich, Joseph Irlin, Michael Kostykov, Amos Fein, Vladimir Toder

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

In the present study, we tried to detect nucleated erythrocytes in maternal blood by means of the HAE9 antibody that binds to the antigen of erythroblasts (Ag-Eb) on nucleated erythroid cells. Umbilical cord blood and placental cells, which are known to contain a high number of nucleated erythrocytes, indeed demonstrated a high percentage of Ag-Eb-positive cells. In comparison, HAE9 stained up to 4.5% of the peripheral blood cells collected from pregnant women, but not those of nonpregnant women or normal men. These results, demonstrating a simple and reliable method for the identification of nucleated erythrocytes in the maternal circulation using the anti-Ag-Eb antibody, might open new possibilities for the detection of nucleated erythrocytes in maternal blood.

Original language	English
Pages (from-to)	126-130
Number of pages	5
Journal	Neonatology
Volume	71
Issue number	2
DOIs	https://doi.org/10.1159/000244406
State	Published - 1997

Keywords

Erythroblast antigen (Ag-Eb)
Flow cytometry
HAE9 antibody
Nucleated erythrocytes

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10.1159/000244406

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title = "Use of Antibodies against the Human Antigen of Erythroblasts for the Detection of Nucleated Erythrocytes in the Maternal Circulation",

abstract = "In the present study, we tried to detect nucleated erythrocytes in maternal blood by means of the HAE9 antibody that binds to the antigen of erythroblasts (Ag-Eb) on nucleated erythroid cells. Umbilical cord blood and placental cells, which are known to contain a high number of nucleated erythrocytes, indeed demonstrated a high percentage of Ag-Eb-positive cells. In comparison, HAE9 stained up to 4.5\% of the peripheral blood cells collected from pregnant women, but not those of nonpregnant women or normal men. These results, demonstrating a simple and reliable method for the identification of nucleated erythrocytes in the maternal circulation using the anti-Ag-Eb antibody, might open new possibilities for the detection of nucleated erythrocytes in maternal blood.",

keywords = "Erythroblast antigen (Ag-Eb), Flow cytometry, HAE9 antibody, Nucleated erythrocytes",

author = "Shoshana Savion and Howard Carp and Jeanne Shepshelovich and Joseph Irlin and Michael Kostykov and Amos Fein and Vladimir Toder",

year = "1997",

doi = "10.1159/000244406",

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T1 - Use of Antibodies against the Human Antigen of Erythroblasts for the Detection of Nucleated Erythrocytes in the Maternal Circulation

AU - Savion, Shoshana

AU - Carp, Howard

AU - Shepshelovich, Jeanne

AU - Irlin, Joseph

AU - Kostykov, Michael

AU - Fein, Amos

AU - Toder, Vladimir

PY - 1997

Y1 - 1997

N2 - In the present study, we tried to detect nucleated erythrocytes in maternal blood by means of the HAE9 antibody that binds to the antigen of erythroblasts (Ag-Eb) on nucleated erythroid cells. Umbilical cord blood and placental cells, which are known to contain a high number of nucleated erythrocytes, indeed demonstrated a high percentage of Ag-Eb-positive cells. In comparison, HAE9 stained up to 4.5% of the peripheral blood cells collected from pregnant women, but not those of nonpregnant women or normal men. These results, demonstrating a simple and reliable method for the identification of nucleated erythrocytes in the maternal circulation using the anti-Ag-Eb antibody, might open new possibilities for the detection of nucleated erythrocytes in maternal blood.

AB - In the present study, we tried to detect nucleated erythrocytes in maternal blood by means of the HAE9 antibody that binds to the antigen of erythroblasts (Ag-Eb) on nucleated erythroid cells. Umbilical cord blood and placental cells, which are known to contain a high number of nucleated erythrocytes, indeed demonstrated a high percentage of Ag-Eb-positive cells. In comparison, HAE9 stained up to 4.5% of the peripheral blood cells collected from pregnant women, but not those of nonpregnant women or normal men. These results, demonstrating a simple and reliable method for the identification of nucleated erythrocytes in the maternal circulation using the anti-Ag-Eb antibody, might open new possibilities for the detection of nucleated erythrocytes in maternal blood.

KW - Erythroblast antigen (Ag-Eb)

KW - Flow cytometry

KW - HAE9 antibody

KW - Nucleated erythrocytes

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Use of Antibodies against the Human Antigen of Erythroblasts for the Detection of Nucleated Erythrocytes in the Maternal Circulation

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Keywords

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