Use of a specific monoclonal antibody for the preparation of a highly active immobilized carboxypeptidase A

Beka Solomon*, Rela Koppel, Ephraim Katchalski-Katzir

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A mouse monoclonal antibody (Ab 100) to carboxypeptidase A (CPA) which binds to the enzyme with a high affinity constant (˜109 M-1) without affecting its catalytic activity was prepared, purified and characterized. Covalent binding of the monoclonal antibody to Eupergit C, or noncovalent binding to Sepharose-Protein A, yielded carriers reacting specifically with CPA to give immobilized enzyme preparations displaying full catalytic activity and improved stability. The method represents a general technique for the preparation of highly active immobilized enzymes.

Original languageEnglish
Pages (from-to)709-712
Number of pages4
JournalBio/Technology
Volume2
Issue number8
DOIs
StatePublished - Aug 1984

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