Use of a novel system for defining a gene imprinting region

Jacob Rachmilewitz, Bernard Gonik, Ran Goshen, Iiana Ariel, Tamar Schneider, Nathan Degroot, Abraham Hochberg*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Gene imprinting involves the expression of a single allele, depending on its parental origin. In this report, we describe the use of a novel system, implementing human tissues exclusively endowed with either maternally or paternally inherited chromosomes, to better define a known gene imprinting region. Specific RNA transcripts for Placental Ribonuclease Inhibitor (PRI) and Cathepsin D were analysed by northern blotting for expression in complete hydatidiform mole, mature teratoma, and normal placenta. These genes are in close proximity to the reciprocally imprinted H19 and IGF-2 genes found on chromosome 11p15.5. Since all the tissues studied expressed Cathepsin D and PAl, these are not, by definition, imprinted, but as yet we cannot define the borders of the imprinting area on chromosome 11p15.5.

Original languageEnglish
Pages (from-to)659-664
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - 29 Oct 1993
Externally publishedYes


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