Usage of Silicon for Label-Free Super-Resolved Imaging

Hadar Pinhas, Yossef Danan, Amihai Meiri, Omer Wagner, Asaf Shahmoon, Tali Ilovitsh, Yehonatan Ramon, Dror Malka, Meir Danino, Moshe Sinvani, Zeev Zalevsky

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


In this chapter, we present a summary of research that uses silicon to enhance the imaging resolution and to push it toward the region of nanoscopy. The silicon has a nonlinear effect called the plasma dispersionDispersioneffect (PDEPlasma Dispersion Effect (PDE)), which can be used instead of the fluorescent dyeFluorescent dyein order to realize a stimulated emission depletion (STED)Stimulated Emission Depletion (STED)like microscopic imaging configuration. The silicon can be encapsulated into nanoparticles while the encapsulation may be biocompatible and yield the first step toward label-freeLabel freebioimaging. The encapsulation may even be metallic to enhance the obtainable effect and to yield resolution enhancement at lower requirements from the pumpPumpbeamPump beam. The silicon can even be used directly as a waferWaferbut then the super-resolving imaging is mainly aimed at failure analysis of micro-electronic circuitry. When the silicon is encapsulated with metallic layer or when it is in the form of a wafer, while being illuminated with a pumpPumpbeamPump beam(inVisible wavelengthsvisible wavelengthWavelengthbeing absorbed by the silicon) it modifies the imaging point spreadFunctionpoint spread (PSF)function (PSF) of the probeProbebeamProbe beam(a near infra-red wavelength) into a doughnutDoughnut-like shape, through the utilization of the PDEPlasma Dispersion Effect (PDE). This modified PSF has spatial components smaller than the diffraction limitDiffraction limit, and by scanning the sample with itSuper resolution, super resolutionResolutionsuper-can be achieved.
Original languageEnglish
Title of host publicationLabel-Free Super-Resolution Microscopy
EditorsVasily Astratov
Place of PublicationCham
PublisherSpringer International Publishing AG
Number of pages25
ISBN (Electronic)978-3-030-21722-8
ISBN (Print)978-3-030-21721-1, 978-3-030-21724-2
StatePublished - 2019
Externally publishedYes

Publication series

NameBiological and Medical Physics, Biomedical Engineering
PublisherSpringer, Cham
ISSN (Print)1618-7210
ISSN (Electronic)2197-5647


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