Unsaturated fatty acids stimulate NADPH-dependent superoxide production by cell-free system derived from macrophages

Yael Bromberg; Edgar Pick

doi:10.1016/0008-8749(84)90066-2

Unsaturated fatty acids stimulate NADPH-dependent superoxide production by cell-free system derived from macrophages

Yael Bromberg, Edgar Pick^*

^*Corresponding author for this work

Clinical Microbiology and Immunology

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

292 Scopus citations

Abstract

Arachidonic acid (C20:4) and other unsaturated fatty acids are shown to activate superoxide (O₂^-) production in a cell-free system represented by sonically disrupted guinea pig peritoneal macrophages. The reaction requires a heat-sensitive cellular component and NADPH, is enhanced by flavin adenine dinucleotide (FAD), and is not linked to enzymatic oxidation of the fatty acid. C20:4-elicited O₂^- formation is dependent on the cooperation between a subcellular component sedimentable at 48,000g (probably containing the O₂^--forming enzyme) and a cytosolic factor. This appears to be the first report of O₂^- generation being elicited in a cell-free system derived from unstimulated cells and supports the idea that unesterified unsaturated fatty acids act as second messengers of O₂^- formation in intact phagocytes.

Original language	English
Pages (from-to)	213-221
Number of pages	9
Journal	Cellular Immunology
Volume	88
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(84)90066-2
State	Published - 1 Oct 1984

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abstract = "Arachidonic acid (C20:4) and other unsaturated fatty acids are shown to activate superoxide (O2-) production in a cell-free system represented by sonically disrupted guinea pig peritoneal macrophages. The reaction requires a heat-sensitive cellular component and NADPH, is enhanced by flavin adenine dinucleotide (FAD), and is not linked to enzymatic oxidation of the fatty acid. C20:4-elicited O2- formation is dependent on the cooperation between a subcellular component sedimentable at 48,000g (probably containing the O2--forming enzyme) and a cytosolic factor. This appears to be the first report of O2- generation being elicited in a cell-free system derived from unstimulated cells and supports the idea that unesterified unsaturated fatty acids act as second messengers of O2- formation in intact phagocytes.",

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AB - Arachidonic acid (C20:4) and other unsaturated fatty acids are shown to activate superoxide (O2-) production in a cell-free system represented by sonically disrupted guinea pig peritoneal macrophages. The reaction requires a heat-sensitive cellular component and NADPH, is enhanced by flavin adenine dinucleotide (FAD), and is not linked to enzymatic oxidation of the fatty acid. C20:4-elicited O2- formation is dependent on the cooperation between a subcellular component sedimentable at 48,000g (probably containing the O2--forming enzyme) and a cytosolic factor. This appears to be the first report of O2- generation being elicited in a cell-free system derived from unstimulated cells and supports the idea that unesterified unsaturated fatty acids act as second messengers of O2- formation in intact phagocytes.

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Unsaturated fatty acids stimulate NADPH-dependent superoxide production by cell-free system derived from macrophages

Abstract

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