TY - JOUR
T1 - Ultraviolet light-resistant primary transfectants of xeroderma pigmentosum cells are also DNA repair-proficient
AU - Stark, Merav
AU - Naiman, Tova
AU - Canaani, Dan
N1 - Funding Information:
This research was supported by grants awarded to D.C. by the Fund for Basic Research administered by the Israel Academy of Sciences and Humanities, the Israel Cancer Research Fund and the United States-Israel Binational Science Foundation (BSF), Jerusalem, Israel. D.C. was also supported by a fellowship from the Hareli Cancer Research Fund.
PY - 1989/8/15
Y1 - 1989/8/15
N2 - In a previous work, an immortal xeroderma pigmentosum cell line belonging to complementation group C was complemented to a UV-resistant phenotype by transfection with a human cDNA clone library. We now report that the primary transformants selected for UV-resistance also acquired normal levels of DNA repair. This was assessed both by measurement of UV-induced [3H]thymidine incorporation and by equilibrium sedimentation analysis of repair-DNA synthesis. Therefore, the transduced DNA element which confers normal UV-resistance also corrects the excision repair defect of the xeroderma pigmentosum group C cell line.
AB - In a previous work, an immortal xeroderma pigmentosum cell line belonging to complementation group C was complemented to a UV-resistant phenotype by transfection with a human cDNA clone library. We now report that the primary transformants selected for UV-resistance also acquired normal levels of DNA repair. This was assessed both by measurement of UV-induced [3H]thymidine incorporation and by equilibrium sedimentation analysis of repair-DNA synthesis. Therefore, the transduced DNA element which confers normal UV-resistance also corrects the excision repair defect of the xeroderma pigmentosum group C cell line.
UR - http://www.scopus.com/inward/record.url?scp=0024325970&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(89)90822-X
DO - 10.1016/0006-291X(89)90822-X
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AN - SCOPUS:0024325970
SN - 0006-291X
VL - 162
SP - 1351
EP - 1356
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -