TY - JOUR
T1 - Ultrastructural characterization of human granulosa cells in stimulated cycles
T2 - Correlation with oocyte fertilizability
AU - Rotmensch, S.
AU - Dor, J.
AU - Furman, A.
AU - Rudak, E.
AU - Mashiach, S.
AU - Amsterdam, A.
N1 - Funding Information:
Received July 10, 1985; revised and accepted January 16, 1986. *Supported by a grant from the Rockefeller Foundation, New York, NY. tDepartment of Hormone Research, The Weizmann Institute of Science. :j:Reprint requests: Siegfried Rotmensch, M.D., Department of Hormone Research, The Weizmann Institute of Science, Rehovot 76100, Israel. §Department of Obstetrics and Gynecology, The Chaim Sheba Medical Center. IIIncumbent of the Joyce and Ben B. Eisenberg Professorial Chair in Molecular Endocrinology and Cancer Research.
PY - 1986
Y1 - 1986
N2 - The fine structure of human granulosa cells (GC) obtained in the course of an in vitro fertilization procedure was correlated with oocyte fertilizability. The investigation was performed by light-microscopic and electron-microscopic examination of ultrathin sections and replicas of freeze-fractured material. GC associated with nonfertilizable oocytes (group I) had significantly smaller cell areas, tended to be tightly packed, and exhibited abundant intercellular gap junctions and adherence junctions. Multiple structural variations of cytoplasmic organelles were observed in comparison to cells associated with fertilizable oocytes (group II). Cells in group II tended to be widely dispersed, frequently contained interiorized gap junctional elements (annular junctions), and showed morphologic correlates of high steroidogenic activity. Structural dissimilarities between groups I and II were not consistently related to follicle size, as determined by volume of aspirated follicular fluid. In view of the sequential ultrastructural changes known to occur during follicular maturation and ovulation, it is concluded that GCs related to nonfertilizable oocytes were lacking structural correlates of luteinizing hormone/human chorionic gonadotropin responsiveness.
AB - The fine structure of human granulosa cells (GC) obtained in the course of an in vitro fertilization procedure was correlated with oocyte fertilizability. The investigation was performed by light-microscopic and electron-microscopic examination of ultrathin sections and replicas of freeze-fractured material. GC associated with nonfertilizable oocytes (group I) had significantly smaller cell areas, tended to be tightly packed, and exhibited abundant intercellular gap junctions and adherence junctions. Multiple structural variations of cytoplasmic organelles were observed in comparison to cells associated with fertilizable oocytes (group II). Cells in group II tended to be widely dispersed, frequently contained interiorized gap junctional elements (annular junctions), and showed morphologic correlates of high steroidogenic activity. Structural dissimilarities between groups I and II were not consistently related to follicle size, as determined by volume of aspirated follicular fluid. In view of the sequential ultrastructural changes known to occur during follicular maturation and ovulation, it is concluded that GCs related to nonfertilizable oocytes were lacking structural correlates of luteinizing hormone/human chorionic gonadotropin responsiveness.
UR - http://www.scopus.com/inward/record.url?scp=0022612532&partnerID=8YFLogxK
U2 - 10.1016/S0015-0282(16)49340-4
DO - 10.1016/S0015-0282(16)49340-4
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AN - SCOPUS:0022612532
SN - 0015-0282
VL - 45
SP - 671
EP - 679
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 5
ER -