Ultrasound-Mediated Targeted Drug Delivery Generated by Multifocal Beam Patterns: An In vitro Study

Dana Gourevich, Yoni Hertzberg, Alexander Volovick, Yaron Shafran, Gil Navon, Sandy Cochran, Andreas Melzer

Research output: Contribution to journalArticlepeer-review

Abstract

Ultrasound-mediated targeted drug delivery has been a subject for a dedicated research activity for several decades. Nevertheless, in vitro studies in this field of research are characterized by their inconsistencies. To improve the repeatability of such experiments, a novel approach of multifocal spot generation was investigated. A multifocal pattern of 16 spots was utilized using an iterative Gerchberg-Saxton algorithm. The pattern was applied to insonate a 96-well Petri dish plate using a clinically available planar-phased array transducer with approximately 1000 elements with a central frequency of 0.55 MHz. The pattern was acoustically characterized and applied to a monolayer of human breast cancer cell line in the 96-well plate. With the help of ultrasonic contrast agents, the intracellular drug uptake was increased by an average factor of 3.5 compared with the control group.

Original languageEnglish
Pages (from-to)507-514
Number of pages8
JournalUltrasound in Medicine and Biology
Volume39
Issue number3
DOIs
StatePublished - Mar 2013

Keywords

  • Cancer cell lines
  • Focused ultrasound
  • Microbubbles
  • Multifocus beam patterns
  • Targeted drug delivery

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