Ultrasound-Mediated Targeted Drug Delivery Generated by Multifocal Beam Patterns: An In vitro Study

Dana Gourevich; Yoni Hertzberg; Alexander Volovick; Yaron Shafran; Gil Navon; Sandy Cochran; Andreas Melzer

doi:10.1016/j.ultrasmedbio.2012.10.013

Ultrasound-Mediated Targeted Drug Delivery Generated by Multifocal Beam Patterns: An In vitro Study

Dana Gourevich, Yoni Hertzberg, Alexander Volovick^*, Yaron Shafran, Gil Navon, Sandy Cochran, Andreas Melzer

^*Corresponding author for this work

School of Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Ultrasound-mediated targeted drug delivery has been a subject for a dedicated research activity for several decades. Nevertheless, in vitro studies in this field of research are characterized by their inconsistencies. To improve the repeatability of such experiments, a novel approach of multifocal spot generation was investigated. A multifocal pattern of 16 spots was utilized using an iterative Gerchberg-Saxton algorithm. The pattern was applied to insonate a 96-well Petri dish plate using a clinically available planar-phased array transducer with approximately 1000 elements with a central frequency of 0.55 MHz. The pattern was acoustically characterized and applied to a monolayer of human breast cancer cell line in the 96-well plate. With the help of ultrasonic contrast agents, the intracellular drug uptake was increased by an average factor of 3.5 compared with the control group.

Original language	English
Pages (from-to)	507-514
Number of pages	8
Journal	Ultrasound in Medicine and Biology
Volume	39
Issue number	3
DOIs	https://doi.org/10.1016/j.ultrasmedbio.2012.10.013
State	Published - Mar 2013

Keywords

Cancer cell lines
Focused ultrasound
Microbubbles
Multifocus beam patterns
Targeted drug delivery

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ultrasmedbio.2012.10.013

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title = "Ultrasound-Mediated Targeted Drug Delivery Generated by Multifocal Beam Patterns: An In vitro Study",

abstract = "Ultrasound-mediated targeted drug delivery has been a subject for a dedicated research activity for several decades. Nevertheless, in vitro studies in this field of research are characterized by their inconsistencies. To improve the repeatability of such experiments, a novel approach of multifocal spot generation was investigated. A multifocal pattern of 16 spots was utilized using an iterative Gerchberg-Saxton algorithm. The pattern was applied to insonate a 96-well Petri dish plate using a clinically available planar-phased array transducer with approximately 1000 elements with a central frequency of 0.55 MHz. The pattern was acoustically characterized and applied to a monolayer of human breast cancer cell line in the 96-well plate. With the help of ultrasonic contrast agents, the intracellular drug uptake was increased by an average factor of 3.5 compared with the control group.",

keywords = "Cancer cell lines, Focused ultrasound, Microbubbles, Multifocus beam patterns, Targeted drug delivery",

author = "Dana Gourevich and Yoni Hertzberg and Alexander Volovick and Yaron Shafran and Gil Navon and Sandy Cochran and Andreas Melzer",

note = "Funding Information: The research leading to these results received funding from the European Community's Seventh Framework Programme ( FP7/2007-2013 ) under grant agreement no. 230674 (Nanoporation project). The authors thank O. Dogadkin, J. Grienfield, O. Prus and Y. Medan of InSightec Ltd.; J. Gnaim of Capsutech Ltd.; and L. Wang of the University of Dundee for their support and assistance in this research.",

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AU - Shafran, Yaron

AU - Navon, Gil

AU - Cochran, Sandy

AU - Melzer, Andreas

N1 - Funding Information: The research leading to these results received funding from the European Community's Seventh Framework Programme ( FP7/2007-2013 ) under grant agreement no. 230674 (Nanoporation project). The authors thank O. Dogadkin, J. Grienfield, O. Prus and Y. Medan of InSightec Ltd.; J. Gnaim of Capsutech Ltd.; and L. Wang of the University of Dundee for their support and assistance in this research.

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AB - Ultrasound-mediated targeted drug delivery has been a subject for a dedicated research activity for several decades. Nevertheless, in vitro studies in this field of research are characterized by their inconsistencies. To improve the repeatability of such experiments, a novel approach of multifocal spot generation was investigated. A multifocal pattern of 16 spots was utilized using an iterative Gerchberg-Saxton algorithm. The pattern was applied to insonate a 96-well Petri dish plate using a clinically available planar-phased array transducer with approximately 1000 elements with a central frequency of 0.55 MHz. The pattern was acoustically characterized and applied to a monolayer of human breast cancer cell line in the 96-well plate. With the help of ultrasonic contrast agents, the intracellular drug uptake was increased by an average factor of 3.5 compared with the control group.

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Ultrasound-Mediated Targeted Drug Delivery Generated by Multifocal Beam Patterns: An In vitro Study

Abstract

Keywords

UN SDGs

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