Ultra-short laser pulses as a tool to measure as well as perturb neurovascular activity in the rodent brain

Philbert S. Tsai, Pablo Blinder, Benjamin Migliori, David Kleinfeld

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The ability to image and perturb brain tissue that lies deep to the surface of cortex has facilitated the solution of old problems in blood flow dynamics and has begun to provide new insights into neurovascular coupling. We summarize the use of ultra-short laser pulses to probe the structure and function of vasodynamics through two-photon laser scanning microscopy in junction with fluorescent labels of blood serum and tissue. We next describe the emerging use of amplified ultra-short laser pulses for plasma-assisted ablation as a means to perturb either blood flow or neuronal activity deep to the surface. Lastly, we discuss the use of these same tools for highthroughput block-face imaging of histological issue, in particular, the complete reconstruction of cortical vasculature.

Original languageEnglish
Title of host publicationFrontiers in Optics, FiO 2008
PublisherOptical Society of America (OSA)
ISBN (Print)9781557528612
DOIs
StatePublished - 2008
Externally publishedYes
EventFrontiers in Optics, FiO 2008 - Rochester, NY, United States
Duration: 19 Oct 200823 Oct 2008

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701

Conference

ConferenceFrontiers in Optics, FiO 2008
Country/TerritoryUnited States
CityRochester, NY
Period19/10/0823/10/08

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