Turning mannitol-rich agricultural waste to poly(3-hydroxybutyrate) with Cobetia amphilecti fermentation and recovery with methyl levulinate as a green solvent

Rima Gnaim, Razan Unis, Nabeel Gnayem, Jagadish Das, Michael Gozin, Alexander Golberg

Research output: Contribution to journalArticlepeer-review

Abstract

The present study explored the use of mannitol and mannitol-rich agro-industrial wastes as substrates for PHB production by Cobetia amphilecti isolated from the green Ulva sp. seaweed. Cultivation of C. amphilecti on mannitol, celery, and olive leaves (OLs) waste led to 4.20, 6.00, and 5.16 g L−1 of cell dry mass (CDM), 76.3, 25.5, and 12.0% of PHB content in CDM and 3.2, 1.53, and 0.62 g L−1 of PHB concentration, respectively; which suggested that they can be exploited as carbon substrates for the production of PHB. Extraction of PHB from C. amphilecti cultures by solubilization in the green solvent methyl levulinate (ML) (2% w/w, 140 °C, 1 h) indicated that the recovery yield and purity of PHB are above 97 and 90% w/w, respectively. The use of ML could be an attractive method for the recovery of PHB when safe and non-toxic solvents are required.

Original languageEnglish
Article number127075
JournalBioresource Technology
Volume352
DOIs
StatePublished - May 2022

Keywords

  • Cobetia amphilecti
  • Green solvent
  • Mannitol-rich agriculture waste
  • Methyl levulinate
  • Poly(3-hydroxybutyrate)

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