TY - JOUR
T1 - Tumor DNA content as a prognostic feature in advanced epithelial ovarian carcinoma
AU - Blumenfeld, Doron
AU - Braly, Patricia S.
AU - Ben-Ezra, Jonathan
AU - Klevecz, Robert R.
PY - 1987/7
Y1 - 1987/7
N2 - Tumor DNA content (ploidy) was determined in 84 patients with epithelial ovarian carcinoma, Stage II-IV. A total of 251 DNA histograms generated by flow cytometry on cells derived from paraffin-embedded specimens were analyzed retrospectively. Of the 84 patients, 44 had tumors which were aneuploid, whereas 33 had diploid, and 7 had tetraploid tumors. Cox regression analysis revealed that age (P < 0.001), stage (P < 0.001), and ploidy (P < 0.001) were independent prognostic features. The median survival time was 19 months and 48 months, respectively, in aneuploid and euploid tumors (P < 0.001). The size of residual after surgery lost its significance when corrected for stage. Multivariate analysis in Stage III tumors revealed that ploidy was the most important prognostic factor (P < 0.001) followed by age (P < 0.025). A remarkable stability of cellular DNA content was found when the primary tumor was compared to the following groups: (1) various metastatic specimens from the primary operation in the same patient; (2) specimens analyzed sequentially from primary, secondary, and tertiary exploratory laparotomy; and (3) peritoneal washings before and after intraperitoneal chemotherapy.
AB - Tumor DNA content (ploidy) was determined in 84 patients with epithelial ovarian carcinoma, Stage II-IV. A total of 251 DNA histograms generated by flow cytometry on cells derived from paraffin-embedded specimens were analyzed retrospectively. Of the 84 patients, 44 had tumors which were aneuploid, whereas 33 had diploid, and 7 had tetraploid tumors. Cox regression analysis revealed that age (P < 0.001), stage (P < 0.001), and ploidy (P < 0.001) were independent prognostic features. The median survival time was 19 months and 48 months, respectively, in aneuploid and euploid tumors (P < 0.001). The size of residual after surgery lost its significance when corrected for stage. Multivariate analysis in Stage III tumors revealed that ploidy was the most important prognostic factor (P < 0.001) followed by age (P < 0.025). A remarkable stability of cellular DNA content was found when the primary tumor was compared to the following groups: (1) various metastatic specimens from the primary operation in the same patient; (2) specimens analyzed sequentially from primary, secondary, and tertiary exploratory laparotomy; and (3) peritoneal washings before and after intraperitoneal chemotherapy.
UR - http://www.scopus.com/inward/record.url?scp=0023376640&partnerID=8YFLogxK
U2 - 10.1016/0090-8258(87)90264-2
DO - 10.1016/0090-8258(87)90264-2
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C2 - 3623235
AN - SCOPUS:0023376640
SN - 0090-8258
VL - 27
SP - 389
EP - 398
JO - Gynecologic Oncology
JF - Gynecologic Oncology
IS - 3
ER -
