Tumor DNA content as a prognostic feature in advanced epithelial ovarian carcinoma

Doron Blumenfeld, Patricia S. Braly*, Jonathan Ben-Ezra, Robert R. Klevecz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Tumor DNA content (ploidy) was determined in 84 patients with epithelial ovarian carcinoma, Stage II-IV. A total of 251 DNA histograms generated by flow cytometry on cells derived from paraffin-embedded specimens were analyzed retrospectively. Of the 84 patients, 44 had tumors which were aneuploid, whereas 33 had diploid, and 7 had tetraploid tumors. Cox regression analysis revealed that age (P < 0.001), stage (P < 0.001), and ploidy (P < 0.001) were independent prognostic features. The median survival time was 19 months and 48 months, respectively, in aneuploid and euploid tumors (P < 0.001). The size of residual after surgery lost its significance when corrected for stage. Multivariate analysis in Stage III tumors revealed that ploidy was the most important prognostic factor (P < 0.001) followed by age (P < 0.025). A remarkable stability of cellular DNA content was found when the primary tumor was compared to the following groups: (1) various metastatic specimens from the primary operation in the same patient; (2) specimens analyzed sequentially from primary, secondary, and tertiary exploratory laparotomy; and (3) peritoneal washings before and after intraperitoneal chemotherapy.

Original languageEnglish
Pages (from-to)389-398
Number of pages10
JournalGynecologic Oncology
Volume27
Issue number3
DOIs
StatePublished - Jul 1987
Externally publishedYes

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