TY - JOUR
T1 - Tumor-Bound Immunoglobulins
T2 - An In Vivo Phenomenon Of Masked Specificity
AU - Dorval, G.
AU - Witz, I. P.
AU - Klein, E.
N1 - Funding Information:
1 Received May 13, 1975; accepted September 4, 1975. 2 Supported by Public Health Service contract NOI-CB33870 (to E.K.), NOI·CB33859 (to H.W.), and N01·CB43858 (to I.P.W.) from the Division of Cancer Biology and Diagnosis, National Cancer Institute. Supported also by grants from the Swedish Cancer Society and by the Canadian Medical Research Council (to G.D.). 3 Department of Tumor Biology, Karolinska Institute, 104 01 Stockholm 60, Sweden. 4 Present address: Department of Immunology, Royal Victoria Hospital, 687 Pine Ave. W., Montreal, H3A lAI, Canada. 5 Recipient of an American Cancer Society-Eleanor RooseveltInternational Cancer Fellowship awarded by the International Union against Cancer. Present address: Department of Microbiology, The Doctor George S. Wise Life Sciences Center, Tel-Aviv Uni· versity, Tel-Aviv, Israel. 6 Department of Immunology, Uppsala University, Box 562, 751 22 Uppsala 1, Sweden. 7 We thank Dr. George Klein for his helpful discussions, Dr. Rolf Kiessling for his help with YAC cells maintained in vitro, and Miss Berit Olsson and Mrs. Ann Sjolund for their technical assistance. 8 Braslawsky G, Yaakobovicz M, Frensdorff A, et al: In preparation. 9 N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid.
PY - 1976/3
Y1 - 1976/3
N2 - We examined the specificity of the Ig “coating” on murine tumor cells grown in vivo. Cells were treated in vitro for release of cell-bound Ig from a.scites tumors. Such uncoated cells showed an increased expression of tumor-associated antigens and a parallel decrease in intensity of the Ig coat, but displayed no changes in the expressions of other normal membrane antigens. This was shown by a complement-dependent cytotoxicity assay and radioimmunoassay. These changes were attenuated if the tumor originated from animals that had been irradiated before tumor inoculation. No alterations were found with corresponding cells propagated in vitro and submitted to the same treatments. Our findings and others suggest tumor-specific antibodies among the Ig coats detected on tumor cells grown in vivo.
AB - We examined the specificity of the Ig “coating” on murine tumor cells grown in vivo. Cells were treated in vitro for release of cell-bound Ig from a.scites tumors. Such uncoated cells showed an increased expression of tumor-associated antigens and a parallel decrease in intensity of the Ig coat, but displayed no changes in the expressions of other normal membrane antigens. This was shown by a complement-dependent cytotoxicity assay and radioimmunoassay. These changes were attenuated if the tumor originated from animals that had been irradiated before tumor inoculation. No alterations were found with corresponding cells propagated in vitro and submitted to the same treatments. Our findings and others suggest tumor-specific antibodies among the Ig coats detected on tumor cells grown in vivo.
UR - http://www.scopus.com/inward/record.url?scp=0017104303&partnerID=8YFLogxK
U2 - 10.1093/jnci/56.3.523
DO - 10.1093/jnci/56.3.523
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AN - SCOPUS:0017104303
SN - 0027-8874
VL - 56
SP - 523
EP - 527
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 3
ER -
