TY - JOUR
T1 - Tubulin is the target binding site for NAP-related peptides
T2 - ADNF-9, D-NAP, and D-SAL
AU - Holtser-Cochav, Miri
AU - Divinski, Inna
AU - Gozes, Illana
N1 - Funding Information:
These studies were supported by the US-Israel Binational Science Foundation, the Israel Science Foundation, the Lily and Avraham Gildor Chair for the Investigation of Growth Factors, the Dr. Diana and Zelman Elton (Elbaum) Laboratory for Molecular Neuroendocrinology, National Institute on Aging (NIA), National Institute of Child Health and Development (NICHD) (intramural), Institute for the Study of Aging (ISOA), and Allon Therapeutics, Inc.
PY - 2006
Y1 - 2006
N2 - The authors set out to investigate whether NAP-related peptides interact with tubulin at a NAP binding site. Previous studies have shown that the neuroprotective peptide NAP binds to tubulin. As NAP (NAPVSIPQ) shares structural similarities with ADNF-9 (SALLRSIPA), and the all-D-enantiomers, D-NAP and D-SAL, it was hypothesized that all of these peptides compete with NAP-tubulin binding. Using NAP affinity column and extracts from newborn rat brain (cerebral cortex), we now show that the above-mentioned peptides compete with NAP binding to tubulin. The identification of tubulin as a target binding site for NAP-related peptides explains, in part, the broad neuroprotective activity offered by these potent peptides.
AB - The authors set out to investigate whether NAP-related peptides interact with tubulin at a NAP binding site. Previous studies have shown that the neuroprotective peptide NAP binds to tubulin. As NAP (NAPVSIPQ) shares structural similarities with ADNF-9 (SALLRSIPA), and the all-D-enantiomers, D-NAP and D-SAL, it was hypothesized that all of these peptides compete with NAP-tubulin binding. Using NAP affinity column and extracts from newborn rat brain (cerebral cortex), we now show that the above-mentioned peptides compete with NAP binding to tubulin. The identification of tubulin as a target binding site for NAP-related peptides explains, in part, the broad neuroprotective activity offered by these potent peptides.
KW - ADNF
KW - ADNP
KW - D-NAP
KW - D-SAL
KW - NAP
KW - Neuroprotection
KW - Tubulin
KW - VIP
UR - http://www.scopus.com/inward/record.url?scp=33646893677&partnerID=8YFLogxK
U2 - 10.1385/JMN:28:3:303
DO - 10.1385/JMN:28:3:303
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AN - SCOPUS:33646893677
SN - 0895-8696
VL - 28
SP - 303
EP - 307
JO - Journal of Molecular Neuroscience
JF - Journal of Molecular Neuroscience
IS - 3
ER -