Tubulin is the target binding site for NAP-related peptides: ADNF-9, D-NAP, and D-SAL

Miri Holtser-Cochav, Inna Divinski, Illana Gozes*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


The authors set out to investigate whether NAP-related peptides interact with tubulin at a NAP binding site. Previous studies have shown that the neuroprotective peptide NAP binds to tubulin. As NAP (NAPVSIPQ) shares structural similarities with ADNF-9 (SALLRSIPA), and the all-D-enantiomers, D-NAP and D-SAL, it was hypothesized that all of these peptides compete with NAP-tubulin binding. Using NAP affinity column and extracts from newborn rat brain (cerebral cortex), we now show that the above-mentioned peptides compete with NAP binding to tubulin. The identification of tubulin as a target binding site for NAP-related peptides explains, in part, the broad neuroprotective activity offered by these potent peptides.

Original languageEnglish
Pages (from-to)303-307
Number of pages5
JournalJournal of Molecular Neuroscience
Issue number3
StatePublished - 2006


FundersFunder number
Allon Therapeutics, Inc.
Institute for the Study of Aging
US-Israel Binational Science Foundation
National Institute on Aging
National Institute of Child Health and Human Development
Israel Science Foundation


    • ADNF
    • ADNP
    • D-NAP
    • D-SAL
    • NAP
    • Neuroprotection
    • Tubulin
    • VIP


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