Trophoblasts isolated from the maternal circulation: In vitro expansion and potential application in non-invasive prenatal diagnosis

Esther Guetta, Liat Gutstein-Abo, Gad Barkai

Research output: Contribution to journalArticlepeer-review

Abstract

Prenatal diagnosis based on rare fetal cells in maternal blood is currently not a feasible option. An effort was made to improve cell yields by targeting trophoblast cells. After sorting, the HLA-G-positive cell fraction was analyzed directly or after culture. In situ hybridization technology was applied to prove fetal cell source in samples from women carrying a male fetus and to predict gender in samples without previous knowledge of fetal sex. In vitro culture led to a significant increase in fetal cells and accurate gender prediction in 93% of these samples. This approach might be useful for non-invasive prenatal diagnosis.

Original languageEnglish
Pages (from-to)337-339
Number of pages3
JournalJournal of Histochemistry and Cytochemistry
Volume53
Issue number3
DOIs
StatePublished - Mar 2005
Externally publishedYes

Keywords

  • Cell sorting
  • Fluorescence in situ
  • Hybridization
  • In vitro culture
  • Prenatal diagnosis
  • Trophoblast cells

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