TY - JOUR
T1 - Transplantation of β cells from transgenic mice into nude athymic diabetic rats restores glucose regulation
AU - Hicks, Barry A.
AU - Stein, Roland
AU - Efrat, Shimon
AU - Grant, Seth
AU - Hanahan, Douglas
AU - Demetriou, Achilles A.
N1 - Funding Information:
Special thanks to Eva Henderson, Andrew Felcher and David Wahoff for technical assistance.T his work was supportedb y grantsf rom the National Institutes of Health (NIDDK) ROl-DK38763-02 and a VeteransA dministration Merit Review Award to A.A.D.; R.S. is the recipient of a Career DevelopmentA ward from the Juvenile Diabetes Foundation. Some of this work has previously been presented in the Proceedingso f the InternationalC ongress,X eno- graft 25, held at Arden House, Harriman, New York in 1988a nd at the meetinge ntitledF rontiers in Diabetes ResearchI I, Lesson from Animal Diabetes III held in Jerusalem, Israel in 1990.
PY - 1991/12
Y1 - 1991/12
N2 - We have shown that elevated plasma d-glucose levels in experimentally-induced diabetic nude athymic rats can be reduced by intraperitoneal transplantation of microcarrier-attached insulin producing β cells from the mouse pancreatic β cell line, β TC-1. The reduction in the level of hyperglycemia was observed as early as two days following cell transplantation and was associated with a concomitant increase in plasma insulin levels. β TC-1 cell transplanted diabetic rats had plasma d-glucose levels similar to those found in non-diabetic control animals and remained normoglycemic throughout the 39 day experimental period. The β TC-1 cell transplanted diabetic rats also had near normalization of body weight, food and water intake and of urine output when compared to control diabetic and non-diabetic rats. Similarly, they exhibited improved blood glucose clearance following intravenous d-glucose administration. These results suggest that β TC-1 cells regulate d-glucose homeostasis following transplantation into diabetic rat recipients in a manner similar to that of endogenous pancreatic β cells.
AB - We have shown that elevated plasma d-glucose levels in experimentally-induced diabetic nude athymic rats can be reduced by intraperitoneal transplantation of microcarrier-attached insulin producing β cells from the mouse pancreatic β cell line, β TC-1. The reduction in the level of hyperglycemia was observed as early as two days following cell transplantation and was associated with a concomitant increase in plasma insulin levels. β TC-1 cell transplanted diabetic rats had plasma d-glucose levels similar to those found in non-diabetic control animals and remained normoglycemic throughout the 39 day experimental period. The β TC-1 cell transplanted diabetic rats also had near normalization of body weight, food and water intake and of urine output when compared to control diabetic and non-diabetic rats. Similarly, they exhibited improved blood glucose clearance following intravenous d-glucose administration. These results suggest that β TC-1 cells regulate d-glucose homeostasis following transplantation into diabetic rat recipients in a manner similar to that of endogenous pancreatic β cells.
KW - Cell therapy
KW - Insulin-dependent diabetes mellitus
KW - Rat
KW - Streptozotocin induced diabetes
KW - β cell transplantation
UR - http://www.scopus.com/inward/record.url?scp=0025719017&partnerID=8YFLogxK
U2 - 10.1016/0168-8227(91)90016-7
DO - 10.1016/0168-8227(91)90016-7
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AN - SCOPUS:0025719017
SN - 0168-8227
VL - 14
SP - 157
EP - 164
JO - Diabetes Research and Clinical Practice
JF - Diabetes Research and Clinical Practice
IS - 3
ER -
