TY - JOUR
T1 - Transmembrane protein structures without X-rays
AU - Fleishman, Sarel J.
AU - Unger, Vinzenz M.
AU - Ben-Tal, Nir
N1 - Funding Information:
We thank I.T. Arkin, D.M. Engelman, S. Harrington, H.R. Kaback and P.L. Sorgen for many useful comments. We regret that, owing to the focus on methods that have been used to predict novel structures, several new approaches are not reviewed. This study was supported by grant 222/04 from the Israel Science Foundation (ISF) to N.B-T., and NIH grants GM66145 and GM071590 to V.M.U. S.J.F. was supported by a doctoral fellowship from the Clore Israel Foundation.
PY - 2006/2
Y1 - 2006/2
N2 - Transmembrane (TM) proteins constitute 15-30% of the genome, but <1% of the structures in the Protein Data Bank. This discrepancy is disturbing, and emphasizes that structure determination of TM proteins remains challenging. The challenge is greatest for proteins from eukaryotes, the structures of which remain intractable despite tremendous advances that have been made towards structure determination of bacterial TM proteins. Notably, >50% of the membrane protein families in eukaryotes lack bacterial homologs. Therefore, it is conceivable that many more years will elapse before high-resolution structures of eukaryotic TM proteins emerge. Until then, integrated approaches that combine biochemical and computational analyses with low-resolution structures are likely to have increasingly important roles in providing frameworks for the mechanistic understanding of membrane-protein structure and function.
AB - Transmembrane (TM) proteins constitute 15-30% of the genome, but <1% of the structures in the Protein Data Bank. This discrepancy is disturbing, and emphasizes that structure determination of TM proteins remains challenging. The challenge is greatest for proteins from eukaryotes, the structures of which remain intractable despite tremendous advances that have been made towards structure determination of bacterial TM proteins. Notably, >50% of the membrane protein families in eukaryotes lack bacterial homologs. Therefore, it is conceivable that many more years will elapse before high-resolution structures of eukaryotic TM proteins emerge. Until then, integrated approaches that combine biochemical and computational analyses with low-resolution structures are likely to have increasingly important roles in providing frameworks for the mechanistic understanding of membrane-protein structure and function.
UR - https://www.scopus.com/pages/publications/32344450353
U2 - 10.1016/j.tibs.2005.12.005
DO - 10.1016/j.tibs.2005.12.005
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AN - SCOPUS:32344450353
SN - 0968-0004
VL - 31
SP - 106
EP - 113
JO - Trends in Biochemical Sciences
JF - Trends in Biochemical Sciences
IS - 2
ER -