Transient deprotonation of bacterial halorhodopsin by photoexcited base

M. Gutman*, E. Nachliel

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The excited singlet state of 6-methoxyquinoline is basic enough to abstract a proton from water and generate OH-. Pulse excitation of such a solution undergoes a massive transient alkalinization. This procedure was employed to monitor the deprotonation of the Schiff base of halorhodopsin. Both the deprotonation and reprotonation of the chromophore are diffusion controlled reactions.

Original languageEnglish
Pages (from-to)29-32
Number of pages4
JournalFEBS Letters
Volume190
Issue number1
DOIs
StatePublished - 7 Oct 1985

Funding

FundersFunder number
American Israeli Binational ScienceF oundation
Israeli Binational ScienceF oundation 3101/823101/82

    Keywords

    • 6-Methoxyquinoline
    • Difffusion-controlled reaction
    • Excited state proton transfer
    • Halorhodopsin
    • pH jump

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