TY - JOUR
T1 - Transgenic organisms expressing genes from Bacillus thuringiensis to combat insect pests
AU - Zaritsky, Arieh
AU - Ben-Dov, Eitan
AU - Borovsky, Dov
AU - Boussiba, Sammy
AU - Einav, Monica
AU - Gindin, Galina
AU - Rami Horowitz, A.
AU - Kolot, Mikhail
AU - Melnikov, Olga
AU - Mendel, Zvi
AU - Yagil, Ezra
N1 - Funding Information:
The research projects described here have been supported by three grants from the United States-Israel Binational Science Foundation, Jerusalem, Israel (#s 2001-042 and 2007-037 to A.Z., E.B-D., D.B. and S.B., and 2003-394 to E.Y. and M.K.), a grant from the Israel Ministry of Agriculture (# 131-1481-09 to A.Z., E.B.D., Z.M. and G.G.), and an Eshkol Fellowship from the Israel Ministry of Science, Jerusalem (# 82606101 to O.M.).
PY - 2010
Y1 - 2010
N2 - Various subspecies (ssp.) of Bacillus thuringiensis (Bt) are considered the best agents known so far to control insects, being highly specific and safe, easily mass produced and with long shelf life.1 The para-cryst lline body that is produced during sporulation I the exosporium includes polypeptides named δ-endotoxins, each killing a specific set of insects. The different entomopathogenic toxins of various Bt ssp. can be manipulated genetically in an educated way to construct more efficient transgenic bacteria or plants that express combinations of toxin genes to control pests.2 Joint research projects in our respective laboratories during the last decade demonstrate what can be done by implementing certain ideas using molecular biology with Bt ssp. israelensis (Bti) as a model system. Here, we describe our progress achieved with Gram-negative bacterial species, including cyanobacteria, and some preliminary experiments to form transgenic plants, mainly to control mosquitoes (Diptera), but also a particular Lepidopteran and Coleopteran pest species. In addition, a system is described by which environment-damaging genes can be removed from the recombinants thus alleviating procedures for obtaining permits to release them in nature.
AB - Various subspecies (ssp.) of Bacillus thuringiensis (Bt) are considered the best agents known so far to control insects, being highly specific and safe, easily mass produced and with long shelf life.1 The para-cryst lline body that is produced during sporulation I the exosporium includes polypeptides named δ-endotoxins, each killing a specific set of insects. The different entomopathogenic toxins of various Bt ssp. can be manipulated genetically in an educated way to construct more efficient transgenic bacteria or plants that express combinations of toxin genes to control pests.2 Joint research projects in our respective laboratories during the last decade demonstrate what can be done by implementing certain ideas using molecular biology with Bt ssp. israelensis (Bti) as a model system. Here, we describe our progress achieved with Gram-negative bacterial species, including cyanobacteria, and some preliminary experiments to form transgenic plants, mainly to control mosquitoes (Diptera), but also a particular Lepidopteran and Coleopteran pest species. In addition, a system is described by which environment-damaging genes can be removed from the recombinants thus alleviating procedures for obtaining permits to release them in nature.
KW - Bacillus thuringiensis
KW - Control of insect pest larvae
KW - Environmental considerations
KW - Recombinant organisms
KW - Synergy
KW - δ-endotoxins
UR - http://www.scopus.com/inward/record.url?scp=78449294766&partnerID=8YFLogxK
U2 - 10.4161/bbug.1.5.13087
DO - 10.4161/bbug.1.5.13087
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C2 - 21326834
AN - SCOPUS:78449294766
SN - 1949-1018
VL - 1
SP - 341
EP - 344
JO - Bioengineered Bugs
JF - Bioengineered Bugs
IS - 5
ER -