TY - JOUR
T1 - Transcriptional analysis of the pst operon of Escherichia coli
AU - Aguena, M.
AU - Yagil, E.
AU - Spira, B.
N1 - Funding Information:
Acknowledgements We thank Barry Wanner for sending us bacterial strains. This research was supported by grants 99/09246-3 and 01/05401-6 from Fundac¸ ão de Amparo a Pesquisa do Estado de São Paulo (FAPESP). EY was supported by a grant from the Kurt Lion Foundation.
PY - 2002
Y1 - 2002
N2 - The pst operon of Escherichia coli, which encodes the phosphate-specific transport system, is composed of five genes, pstS, pstC, pstA, pstB and phoU, whose transcription is induced by phosphate starvation. A phosphate-regulated promoter located upstream of the most proximal gene (pstS) controls the transcription of the entire operon. Though the full-length pst mRNA could be detected by an improved RT-PCR protocol, Northern analysis using several pst-specific probes failed to reveal this transcript. Instead, smaller but distinct pst mRNA species were evident. Primer-extension experiments localized the 5′ ends of pst mRNAs within the operon. The data suggest that the full-length mRNA is rapidly processed post-transcriptionally.
AB - The pst operon of Escherichia coli, which encodes the phosphate-specific transport system, is composed of five genes, pstS, pstC, pstA, pstB and phoU, whose transcription is induced by phosphate starvation. A phosphate-regulated promoter located upstream of the most proximal gene (pstS) controls the transcription of the entire operon. Though the full-length pst mRNA could be detected by an improved RT-PCR protocol, Northern analysis using several pst-specific probes failed to reveal this transcript. Instead, smaller but distinct pst mRNA species were evident. Primer-extension experiments localized the 5′ ends of pst mRNAs within the operon. The data suggest that the full-length mRNA is rapidly processed post-transcriptionally.
KW - Escherichia coli
KW - PHO-regulon
KW - pst operon
UR - https://www.scopus.com/pages/publications/0036454109
U2 - 10.1007/s00438-002-0764-4
DO - 10.1007/s00438-002-0764-4
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C2 - 12471449
AN - SCOPUS:0036454109
SN - 1617-4615
VL - 268
SP - 518
EP - 524
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
IS - 4
ER -