Transcription in the nucleus and mRNA decay in the cytoplasm are coupled processes

Vicky Goler-Baron; Michael Selitrennik; Oren Barkai; Gal Haimovich; Rona Lotan; Mordechai Choder

doi:10.1101/gad.473608

Transcription in the nucleus and mRNA decay in the cytoplasm are coupled processes

Vicky Goler-Baron, Michael Selitrennik, Oren Barkai, Gal Haimovich, Rona Lotan, Mordechai Choder^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Maintaining appropriate mRNAs levels is vital for any living cell. mRNA synthesis in the nucleus by RNA polymerase II core enzyme (Pol II) and mRNA decay by cytoplasmic machineries determine these levels. Yet, little is known about possible cross-talk between these processes. The yeast Rpb4/7 is a nucleo-cytoplasmic shuttling heterodimer that interacts with Pol II and with mRNAs and is required for mRNA decay in the cytoplasm. Here we show that interaction of Rpb4/7 with mRNAs and eventual decay of these mRNAs in the cytoplasm depends on association of Rpb4/7 with Pol II in the nucleus. We propose that, following its interaction with Pol II, Rpb4/7 functions in transcription, interacts with the transcript cotranscriptionally and travels with it to the cytoplasm to stimulate mRNA decay. Hence, by recruiting Rpb4/7, Pol II governs not only transcription but also mRNA decay.

Original language	English
Pages (from-to)	2022-2027
Number of pages	6
Journal	Genes and Development
Volume	22
Issue number	15
DOIs	https://doi.org/10.1101/gad.473608
State	Published - 1 Aug 2008
Externally published	Yes

Keywords

RNA polymerase II
Yeast
mRNA decay

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10.1101/gad.473608

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title = "Transcription in the nucleus and mRNA decay in the cytoplasm are coupled processes",

abstract = "Maintaining appropriate mRNAs levels is vital for any living cell. mRNA synthesis in the nucleus by RNA polymerase II core enzyme (Pol II) and mRNA decay by cytoplasmic machineries determine these levels. Yet, little is known about possible cross-talk between these processes. The yeast Rpb4/7 is a nucleo-cytoplasmic shuttling heterodimer that interacts with Pol II and with mRNAs and is required for mRNA decay in the cytoplasm. Here we show that interaction of Rpb4/7 with mRNAs and eventual decay of these mRNAs in the cytoplasm depends on association of Rpb4/7 with Pol II in the nucleus. We propose that, following its interaction with Pol II, Rpb4/7 functions in transcription, interacts with the transcript cotranscriptionally and travels with it to the cytoplasm to stimulate mRNA decay. Hence, by recruiting Rpb4/7, Pol II governs not only transcription but also mRNA decay.",

keywords = "RNA polymerase II, Yeast, mRNA decay",

author = "Vicky Goler-Baron and Michael Selitrennik and Oren Barkai and Gal Haimovich and Rona Lotan and Mordechai Choder",

note = "Funding Information: The authors are grateful for funding received from the Spanish Ministry of Economy and Competitiveness through projects SAF2013/43475-R (MDR) and ISCIII PI14/00931 (FL) and from the Community of Madrid through projects S2010/BMD-2420 (MDR) and S2010/BMD-2359 (FL). ",

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AU - Selitrennik, Michael

AU - Barkai, Oren

AU - Haimovich, Gal

AU - Lotan, Rona

AU - Choder, Mordechai

N1 - Funding Information: The authors are grateful for funding received from the Spanish Ministry of Economy and Competitiveness through projects SAF2013/43475-R (MDR) and ISCIII PI14/00931 (FL) and from the Community of Madrid through projects S2010/BMD-2420 (MDR) and S2010/BMD-2359 (FL).

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N2 - Maintaining appropriate mRNAs levels is vital for any living cell. mRNA synthesis in the nucleus by RNA polymerase II core enzyme (Pol II) and mRNA decay by cytoplasmic machineries determine these levels. Yet, little is known about possible cross-talk between these processes. The yeast Rpb4/7 is a nucleo-cytoplasmic shuttling heterodimer that interacts with Pol II and with mRNAs and is required for mRNA decay in the cytoplasm. Here we show that interaction of Rpb4/7 with mRNAs and eventual decay of these mRNAs in the cytoplasm depends on association of Rpb4/7 with Pol II in the nucleus. We propose that, following its interaction with Pol II, Rpb4/7 functions in transcription, interacts with the transcript cotranscriptionally and travels with it to the cytoplasm to stimulate mRNA decay. Hence, by recruiting Rpb4/7, Pol II governs not only transcription but also mRNA decay.

AB - Maintaining appropriate mRNAs levels is vital for any living cell. mRNA synthesis in the nucleus by RNA polymerase II core enzyme (Pol II) and mRNA decay by cytoplasmic machineries determine these levels. Yet, little is known about possible cross-talk between these processes. The yeast Rpb4/7 is a nucleo-cytoplasmic shuttling heterodimer that interacts with Pol II and with mRNAs and is required for mRNA decay in the cytoplasm. Here we show that interaction of Rpb4/7 with mRNAs and eventual decay of these mRNAs in the cytoplasm depends on association of Rpb4/7 with Pol II in the nucleus. We propose that, following its interaction with Pol II, Rpb4/7 functions in transcription, interacts with the transcript cotranscriptionally and travels with it to the cytoplasm to stimulate mRNA decay. Hence, by recruiting Rpb4/7, Pol II governs not only transcription but also mRNA decay.

KW - RNA polymerase II

KW - Yeast

KW - mRNA decay

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JF - Genes and Development

IS - 15

ER -

Transcription in the nucleus and mRNA decay in the cytoplasm are coupled processes

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Keywords

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