@inproceedings{5933357e382f48db854ac2fa4de9d746,
title = "Toward epigenetic mapping of human chromosomes in nanochannel arrays",
abstract = "We have recently develop a method for optical detection of the epigenetic modification 5-hydroxymethylcytosine (5hmC) at the single-molecule level. We use a chemo-enzymatic reaction to covalently attach a fluorescent reporter molecule to 5hmC nucleotides. Labeled DNA is electro-kinetically squeezed into an array of silicon nanochannels and imaged on a fluorescence microscope. 5hmC residues are visible as fluorescent spots along the DNA contour. Single-molecule imaging of extended lambda phage genomes that are hydroxymethylated at specific sites revealed individual fluorescent labels at expected positions along the genome. We show first results of single-molecule epigenetic mapping of chromosomal DNA extracted from human peripheral blood cells.",
keywords = "Genome mapping, Hydroxymethylcytosine, Nanochannels, Single-molecule",
author = "Y. Michaeli and T. Gabrieli and Y. Ebenstein",
note = "Publisher Copyright: {\textcopyright} 14CBMS.; 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 ; Conference date: 26-10-2014 Through 30-10-2014",
year = "2014",
language = "אנגלית",
series = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",
publisher = "Chemical and Biological Microsystems Society",
pages = "2538--2539",
booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",
}