TY - JOUR
T1 - Topoisomerase IIα expression in ductal carcinoma in situ of the breast
T2 - A preliminary study
AU - Shpitz, Baruch
AU - Bomstein, Yonit
AU - Zehavi, Tania
AU - Bernheim, Joelle
AU - Liverant, Sigal
AU - Kaufman, Zvi
AU - Buklan, Genadi
AU - Klein, Ehud
N1 - Funding Information:
From the Departments of Surgery B and Pathology, Meir General Hospital, affiliated with the Sackler School of Medicine, Tel Aviv University, Israel. Accepted for publication July 6, 2000. Supported by a grant of Israel Cancer Association and Ofer Family donation. Presented at the Annual Meeting of American Association for Cancer Research, New Orleans, LA, 1998. Address correspondence and reprint requests to Baruch Shpitz, MD, Departments of Surgery B and Pathology, Meir General Hospital, Kfar Sava 44281, Affiliated to Sackler School of Medicine, Tel Aviv University, Israel. Copyright © 2000 by W.B. Saunders Company 0046-8177/00/3110-0012$10.00/0 doi:10.1053/hupa.2000.19297
PY - 2000
Y1 - 2000
N2 - Ductal carcinoma in situ (DCIS) of the breast, a precursor lesion of invasive breast cancer, is a heterogeneous disease in terms of histomorphologic features and biologic behavior. Our aim was to assess the Proliferative activity, expressed as topoisomerase IIα (Topo IIα) immunoreactivity and c-erbB-2 expression in relation to morphologic features and architectural pattern of DCIS. The study included 26 DCIS, which were reclassified according to the recommendations of Consensus Conference. Topo-IIα and c-erbB-2 immunoreactivity were detected on paraffin sections. Topo IIα was consistently negative in normal ductal epithelium. Topo IIα-abeling index (Topo IIα-LI) was 0.7 ± 0.6% for grade I, 4.3 ± 3.9% for grade II, and 13.4 ± 8.9 for grade III lesions (P <.01). For mixed nuclear grade DCIS, Topo IIα-LI was 6.8 ± 4.8. There was no difference in Topo IIα-LI between different architectural patterns in low- and intermediate-grade lesions. In high nuclear grade DCIS, there was a progressive increase in Topo IIα-LI from solid toward cribriform and comedo-type DCIS. Positive c-erbB-2 immunoreactivity was found in 46% of DCIS, being highest in DCIS with high nuclear grade (78%) and in lesions with extensive necrosis. Topo IIα-LI was significantly higher in c-erbB-2-positive lesions (Topo IIα-LI- 12.4 ± 8.5) as compared with negative lesions (Topo IIα-LI- 3.9 ± 4.5, P <.0001). Overexpression of c-erbB-2 and Topo IIα is associated with poorly differentiated lesions. Proliferative activity in individual ducts of DCIS depended primarily on the nuclear grade and was independent of architectural patterns of individual ducts in architecturally heterogeneous lesions. (C) 2000 by W.B. Saunders Company.
AB - Ductal carcinoma in situ (DCIS) of the breast, a precursor lesion of invasive breast cancer, is a heterogeneous disease in terms of histomorphologic features and biologic behavior. Our aim was to assess the Proliferative activity, expressed as topoisomerase IIα (Topo IIα) immunoreactivity and c-erbB-2 expression in relation to morphologic features and architectural pattern of DCIS. The study included 26 DCIS, which were reclassified according to the recommendations of Consensus Conference. Topo-IIα and c-erbB-2 immunoreactivity were detected on paraffin sections. Topo IIα was consistently negative in normal ductal epithelium. Topo IIα-abeling index (Topo IIα-LI) was 0.7 ± 0.6% for grade I, 4.3 ± 3.9% for grade II, and 13.4 ± 8.9 for grade III lesions (P <.01). For mixed nuclear grade DCIS, Topo IIα-LI was 6.8 ± 4.8. There was no difference in Topo IIα-LI between different architectural patterns in low- and intermediate-grade lesions. In high nuclear grade DCIS, there was a progressive increase in Topo IIα-LI from solid toward cribriform and comedo-type DCIS. Positive c-erbB-2 immunoreactivity was found in 46% of DCIS, being highest in DCIS with high nuclear grade (78%) and in lesions with extensive necrosis. Topo IIα-LI was significantly higher in c-erbB-2-positive lesions (Topo IIα-LI- 12.4 ± 8.5) as compared with negative lesions (Topo IIα-LI- 3.9 ± 4.5, P <.0001). Overexpression of c-erbB-2 and Topo IIα is associated with poorly differentiated lesions. Proliferative activity in individual ducts of DCIS depended primarily on the nuclear grade and was independent of architectural patterns of individual ducts in architecturally heterogeneous lesions. (C) 2000 by W.B. Saunders Company.
KW - Breast
KW - Ductal carcinoma in sire
KW - Topoisomerase IIα proliferation
UR - http://www.scopus.com/inward/record.url?scp=0033623488&partnerID=8YFLogxK
U2 - 10.1053/hupa.2000.19297
DO - 10.1053/hupa.2000.19297
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0033623488
SN - 0046-8177
VL - 31
SP - 1249
EP - 1254
JO - Human Pathology
JF - Human Pathology
IS - 10
ER -