TY - JOUR
T1 - Topographic changes of membrane receptors for wheat germ agglutinin during the cell cycle and their relation to cytolysis
AU - Lustig, Shlomo
AU - Fishman, Pnina
AU - Djaldetti, Meir
AU - Pluznik, Dov H.
PY - 1980
Y1 - 1980
N2 - Experiments with synchronous cultures of murine mastocytoma cells indicated that cells incubated with wheat germ agglutinin (WGA) responded with cap formation and cytolysis during the mitotic phase only, but not during the G1, S and G2 phases of the cell cycle. The telophase of mitosis was found to be most sensitive to the cytolytic effect of WGA. Addition of the lectin to cells enhanced movement of the lectin-binding sites to the cleavage furrow during the telophase, as demonstrable by the concentration of fluorescent lectin and microvilli in this region. This phenomenon was followed by osmotic lysis of the cells which could be prevented by addition to the medium of dextran of high molecular weight.
AB - Experiments with synchronous cultures of murine mastocytoma cells indicated that cells incubated with wheat germ agglutinin (WGA) responded with cap formation and cytolysis during the mitotic phase only, but not during the G1, S and G2 phases of the cell cycle. The telophase of mitosis was found to be most sensitive to the cytolytic effect of WGA. Addition of the lectin to cells enhanced movement of the lectin-binding sites to the cleavage furrow during the telophase, as demonstrable by the concentration of fluorescent lectin and microvilli in this region. This phenomenon was followed by osmotic lysis of the cells which could be prevented by addition to the medium of dextran of high molecular weight.
UR - http://www.scopus.com/inward/record.url?scp=0019168539&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(80)90499-1
DO - 10.1016/0014-4827(80)90499-1
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C2 - 6775966
AN - SCOPUS:0019168539
SN - 0014-4827
VL - 129
SP - 321
EP - 328
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -