Topographic changes of membrane receptors for wheat germ agglutinin during the cell cycle and their relation to cytolysis

Shlomo Lustig, Pnina Fishman, Meir Djaldetti, Dov H. Pluznik

Research output: Contribution to journalArticlepeer-review

Abstract

Experiments with synchronous cultures of murine mastocytoma cells indicated that cells incubated with wheat germ agglutinin (WGA) responded with cap formation and cytolysis during the mitotic phase only, but not during the G1, S and G2 phases of the cell cycle. The telophase of mitosis was found to be most sensitive to the cytolytic effect of WGA. Addition of the lectin to cells enhanced movement of the lectin-binding sites to the cleavage furrow during the telophase, as demonstrable by the concentration of fluorescent lectin and microvilli in this region. This phenomenon was followed by osmotic lysis of the cells which could be prevented by addition to the medium of dextran of high molecular weight.

Original languageEnglish
Pages (from-to)321-328
Number of pages8
JournalExperimental Cell Research
Volume129
Issue number2
DOIs
StatePublished - 1980
Externally publishedYes

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