TNFα and anti-Fas antibodies regulate Ly-6E.1 expression by tumor cells: A possible link between angiogenesis and Ly-6E.1

Orit Sagi-Assif; Alexandra Traister; Ben Zion Katz; Romema Anavi; Marsel Eskenasy; Isaac P. Witz

doi:10.1016/S0165-2478(96)02675-2

TNFα and anti-Fas antibodies regulate Ly-6E.1 expression by tumor cells: A possible link between angiogenesis and Ly-6E.1

Orit Sagi-Assif, Alexandra Traister, Ben Zion Katz, Romema Anavi, Marsel Eskenasy, Isaac P. Witz^*

^*Corresponding author for this work

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Angiogenic and poorly angiogenic tumor variants were obtained by an intraperitoneal inoculation of cells from clones of polyoma-virus transformed BALB/c 3T3 cells into syngeneic mice. The angiogenic tumor cells expressed a higher tumorigenicity phenotype and a higher capacity to produce artificial pulmonary metastases than cells from the poorly angiogenic tumors. The former cells expressed also significantly higher levels of the lymphocyte activation protein Ly-6E.1 than the former cells. The two types of cells did not differ in expression levels of CD44 and of a polyoma-virus specific membrane antigen. These results raise the possibility that the angiogenic phenotype is coregulated with Ly-6. The effect on Ly-6 expression of signal transduction through TNF receptors, functioning as pivotal regulators of angiogenesis was therefore studied. It was found that TNFα and more so antibodies against Fas down-regulate expression levels of Ly-6. This down-regulation seemed to be selective as expression levels of CD44 were not affected by this treatment.

Original language	English
Pages (from-to)	207-213
Number of pages	7
Journal	Immunology Letters
Volume	54
Issue number	2-3
DOIs	https://doi.org/10.1016/S0165-2478(96)02675-2
State	Published - 2 Dec 1996

Funding

Funders	Funder number
BMFT/DKFZ
Fainbarg Family Fund
German-Israeli Foundation for Scientific Research and Development
United States-Israel Binational Science Foundation

Keywords

Ly-6E.1 expression
TNFα
anti-fas antibodies
tumor cells

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0165-2478(96)02675-2

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title = "TNFα and anti-Fas antibodies regulate Ly-6E.1 expression by tumor cells: A possible link between angiogenesis and Ly-6E.1",

abstract = "Angiogenic and poorly angiogenic tumor variants were obtained by an intraperitoneal inoculation of cells from clones of polyoma-virus transformed BALB/c 3T3 cells into syngeneic mice. The angiogenic tumor cells expressed a higher tumorigenicity phenotype and a higher capacity to produce artificial pulmonary metastases than cells from the poorly angiogenic tumors. The former cells expressed also significantly higher levels of the lymphocyte activation protein Ly-6E.1 than the former cells. The two types of cells did not differ in expression levels of CD44 and of a polyoma-virus specific membrane antigen. These results raise the possibility that the angiogenic phenotype is coregulated with Ly-6. The effect on Ly-6 expression of signal transduction through TNF receptors, functioning as pivotal regulators of angiogenesis was therefore studied. It was found that TNFα and more so antibodies against Fas down-regulate expression levels of Ly-6. This down-regulation seemed to be selective as expression levels of CD44 were not affected by this treatment.",

keywords = "Ly-6E.1 expression, TNFα, anti-fas antibodies, tumor cells",

author = "Orit Sagi-Assif and Alexandra Traister and Katz, {Ben Zion} and Romema Anavi and Marsel Eskenasy and Witz, {Isaac P.}",

note = "Funding Information: We thank Dr Ethan Shevach for his supply of hybridomas. This study was supported by grants from the German-Israeli Foundation for Scientific Research and Development; a joint German-(BMFT/DKFZ) Israeli (MOSA) project; The US-Israel Binational Science Foundation; The Fainbarg Family Fund, Orange-County, CA, USA; and the Pikovsky Fund, Jerusalem, Israel. I.P.W. is the incumbent of the David Furman Chair in Immunobiology of Cancer.",

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doi = "10.1016/S0165-2478(96)02675-2",

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AU - Traister, Alexandra

AU - Katz, Ben Zion

AU - Anavi, Romema

AU - Eskenasy, Marsel

AU - Witz, Isaac P.

N1 - Funding Information: We thank Dr Ethan Shevach for his supply of hybridomas. This study was supported by grants from the German-Israeli Foundation for Scientific Research and Development; a joint German-(BMFT/DKFZ) Israeli (MOSA) project; The US-Israel Binational Science Foundation; The Fainbarg Family Fund, Orange-County, CA, USA; and the Pikovsky Fund, Jerusalem, Israel. I.P.W. is the incumbent of the David Furman Chair in Immunobiology of Cancer.

PY - 1996/12/2

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N2 - Angiogenic and poorly angiogenic tumor variants were obtained by an intraperitoneal inoculation of cells from clones of polyoma-virus transformed BALB/c 3T3 cells into syngeneic mice. The angiogenic tumor cells expressed a higher tumorigenicity phenotype and a higher capacity to produce artificial pulmonary metastases than cells from the poorly angiogenic tumors. The former cells expressed also significantly higher levels of the lymphocyte activation protein Ly-6E.1 than the former cells. The two types of cells did not differ in expression levels of CD44 and of a polyoma-virus specific membrane antigen. These results raise the possibility that the angiogenic phenotype is coregulated with Ly-6. The effect on Ly-6 expression of signal transduction through TNF receptors, functioning as pivotal regulators of angiogenesis was therefore studied. It was found that TNFα and more so antibodies against Fas down-regulate expression levels of Ly-6. This down-regulation seemed to be selective as expression levels of CD44 were not affected by this treatment.

AB - Angiogenic and poorly angiogenic tumor variants were obtained by an intraperitoneal inoculation of cells from clones of polyoma-virus transformed BALB/c 3T3 cells into syngeneic mice. The angiogenic tumor cells expressed a higher tumorigenicity phenotype and a higher capacity to produce artificial pulmonary metastases than cells from the poorly angiogenic tumors. The former cells expressed also significantly higher levels of the lymphocyte activation protein Ly-6E.1 than the former cells. The two types of cells did not differ in expression levels of CD44 and of a polyoma-virus specific membrane antigen. These results raise the possibility that the angiogenic phenotype is coregulated with Ly-6. The effect on Ly-6 expression of signal transduction through TNF receptors, functioning as pivotal regulators of angiogenesis was therefore studied. It was found that TNFα and more so antibodies against Fas down-regulate expression levels of Ly-6. This down-regulation seemed to be selective as expression levels of CD44 were not affected by this treatment.

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TNFα and anti-Fas antibodies regulate Ly-6E.1 expression by tumor cells: A possible link between angiogenesis and Ly-6E.1

Abstract

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Keywords

UN SDGs

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