Time multiplexing super-resolution nanoscopy based on the Brownian motion of gold nanoparticles

Tali Ilovitsh, Asaf Ilovitsh, Omer Wagner, Zeev Zalevsky

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Super-resolution localization microscopy can overcome the diffraction limit and achieve a tens of order improvement in resolution. It requires labeling the sample with fluorescent probes followed with their repeated cycles of activation and photobleaching. This work presents an alternative approach that is free from direct labeling and does not require the activation and photobleaching cycles. Fluorescently labeled gold nanoparticles in a solution are distributed on top of the sample. The nanoparticles move in a random Brownian motion, and interact with the sample. By obscuring different areas in the sample, the nanoparticles encode the sub-wavelength features. A sequence of images of the sample is captured and decoded by digital post processing to create the super-resolution image. The achievable resolution is limited by the additive noise and the size of the nanoparticles. Regular nanoparticles with diameter smaller than 100nm are barely seen in a conventional bright field microscope, thus fluorescently labeled gold nanoparticles were used, with proper modifications for the setup. The method is validated both by numerical simulations as well as by experimental data.

Original languageEnglish
Title of host publicationNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIV
EditorsDan V. Nicolau, Dror Fixler, Alexander N. Cartwright
PublisherSPIE
ISBN (Electronic)9781510605954
DOIs
StatePublished - 2017
Externally publishedYes
EventNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIV 2017 - San Francisco, United States
Duration: 30 Jan 20171 Feb 2017

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10077
ISSN (Print)1605-7422

Conference

ConferenceNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIV 2017
Country/TerritoryUnited States
CitySan Francisco
Period30/01/171/02/17

Keywords

  • Fluorescence
  • Image processing
  • Microscopy
  • Nanoparticles
  • Superresolution

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