Time-dependent cytokine and chemokine changes in mouse cerebral cortex following a mild traumatic brain injury

David Tweedie, Hanuma Kumar Karnati, Roger Mullins, Chaim G. Pick, Barry J. Hoffer, Edward J. Goetzl, Dimitrios Kapogiannis, Nigel H. Greig

Research output: Contribution to journalArticlepeer-review

Abstract

Traumatic brain injury (TBI) is a serious global health problem, many individuals live with TBI-related neurological dysfunction. A lack of biomarkers of TBI has impeded medication development. To identify new potential biomarkers, we time-dependently evaluated mouse brain tissue and neuronally derived plasma extracellular vesicle proteins in a mild model of TBI with parallels to concussive head injury. Mice (CD-1, 30–40 g) received a sham procedure or 30 g weight-drop and were euthanized 8, 24, 48, 72, 96 hr, 7, 14 and 30 days later. We quantified ipsilateral cortical proteins, many of which differed from sham by 8 hours post-mTBI, particularly GAS-1 and VEGF-B were increased while CXCL16 reduced, 23 proteins changed in 4 or more of the time points. Gene ontology pathways mapped from altered proteins over time related to pathological and physiological processes. Validation of proteins identified in this study may provide utility as treatment response biomarkers.

Original languageEnglish
Article numbere55827
Pages (from-to)1-29
Number of pages29
JournaleLife
Volume9
DOIs
StatePublished - Aug 2020

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