TY - JOUR
T1 - Thymidine incorporation into Pharbitis DNA
T2 - II. Adenosine enhancement in vivo; total incorporation versus autoradiography
AU - Zilberstein, Aviah
AU - Arzee, Tova
AU - Gressel, J.
N1 - Funding Information:
The most capable technical assistance of Edna Frenkel and Edna Brick is gratefully acknowledged. This research was supported in part by a grant from the U.S. Department of Agriculture under Public Law 480.
PY - 1973/10
Y1 - 1973/10
N2 - The rate of 3H-methyl thymidine incorporation increased in two waves following floral induction in Pharbitis. Adenosine only enhanced thymidine incorporation at non-peak times. This enhancement was studied by autoradiography and found to be both nuclear and cytoplasmic. Our interpretation of the results is that there was increased thymidine labeling of nuclei already undergoing DNA synthesis and not an increase of cells in S phase. Various vagaries and possible artifacts in the use of thymidine to measure DNA synthesis quantitatively are pointed out.
AB - The rate of 3H-methyl thymidine incorporation increased in two waves following floral induction in Pharbitis. Adenosine only enhanced thymidine incorporation at non-peak times. This enhancement was studied by autoradiography and found to be both nuclear and cytoplasmic. Our interpretation of the results is that there was increased thymidine labeling of nuclei already undergoing DNA synthesis and not an increase of cells in S phase. Various vagaries and possible artifacts in the use of thymidine to measure DNA synthesis quantitatively are pointed out.
UR - http://www.scopus.com/inward/record.url?scp=0015703424&partnerID=8YFLogxK
U2 - 10.1016/0045-6039(73)90009-2
DO - 10.1016/0045-6039(73)90009-2
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0015703424
SN - 0045-6039
VL - 2
SP - 213
EP - 220
JO - Cell Differentiation
JF - Cell Differentiation
IS - 4
ER -