Three-dimensional imaging using phase retrieval with two focus planes

Tali Ilovitsh, Asaf Ilovitsh, Aryeh Weiss, Rinat Meir, Zeev Zalevsky

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

This work presents a technique for a full 3D imaging of biological samples tagged with gold-nanoparticles (GNPs) using only two images, rather than many images per volume as is currently needed for 3D optical sectioning microscopy. The proposed approach is based on the Gerchberg-Saxton (GS) phase retrieval algorithm. The reconstructed field is free space propagated to all other focus planes using post processing, and the 2D z-stack is merged to create a 3D image of the sample with high fidelity. Because we propose to apply the phase retrieving on nano particles, the regular ambiguities typical to the Gerchberg-Saxton algorithm, are eliminated. In addition, since the method requires the capturing of two images only, it can be suitable for 3D live cell imaging. The proposed concept is presented and validated both on simulated data as well as experimentally.

Original languageEnglish
Title of host publicationThree-Dimensional and Multidimensional Microscopy
Subtitle of host publicationImage Acquisition and Processing XXIII
EditorsCarol J. Cogswell, Tony Wilson, Thomas G. Brown
PublisherSPIE
ISBN (Electronic)9781628419474
DOIs
StatePublished - 2016
Externally publishedYes
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII - San Francisco, United States
Duration: 15 Feb 201617 Feb 2016

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9713
ISSN (Print)1605-7422

Conference

ConferenceThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII
Country/TerritoryUnited States
CitySan Francisco
Period15/02/1617/02/16

Keywords

  • Cellular imaging
  • Phase retrieval
  • Three-dimensional microscopy
  • gold nanoparticle

Fingerprint

Dive into the research topics of 'Three-dimensional imaging using phase retrieval with two focus planes'. Together they form a unique fingerprint.

Cite this