Three-dimensional chitin-based scaffolds from Verongida sponges (Demospongiae: Porifera). Part I. Isolation and identification of chitin

H. Ehrlich; M. Ilan; M. Maldonado; G. Muricy; G. Bavestrello; Z. Kljajic; J. L. Carballo; S. Schiaparelli; A. Ereskovsky; P. Schupp; R. Born; H. Worch; V. V. Bazhenov; D. Kurek; V. Varlamov; D. Vyalikh; K. Kummer; V. Sivkov; S. L. Molodtsov; H. Meissner; G. Richter; E. Steck; W. Richter; S. Hunoldt; M. Kammer; S. Paasch; V. Krasokhin; G. Patzke; E. Brunner

doi:10.1016/j.ijbiomac.2010.05.007

Three-dimensional chitin-based scaffolds from Verongida sponges (Demospongiae: Porifera). Part I. Isolation and identification of chitin

H. Ehrlich^*, M. Ilan, M. Maldonado, G. Muricy, G. Bavestrello, Z. Kljajic, J. L. Carballo, S. Schiaparelli, A. Ereskovsky, P. Schupp, R. Born, H. Worch, V. V. Bazhenov, D. Kurek, V. Varlamov, D. Vyalikh, K. Kummer, V. Sivkov, S. L. Molodtsov, H. MeissnerG. Richter, E. Steck, W. Richter, S. Hunoldt, M. Kammer, S. Paasch, V. Krasokhin, G. Patzke, E. Brunner

^*Corresponding author for this work

School of Zoology

Research output: Contribution to journal › Article › peer-review

133 Scopus citations

Abstract

Marine invertebrate organisms including sponges (Porifera) not only provide an abundant source of biologically active secondary metabolites but also inspire investigations to develop biomimetic composites, scaffolds and templates for practical use in materials science, biomedicine and tissue engineering. Here, we presented a detailed study of the structural and physico-chemical properties of three-dimensional skeletal scaffolds of the marine sponges Aiolochroia crassa, Aplysina aerophoba, A. cauliformis, A. cavernicola, and A. fulva (Verongida: Demospongiae). We show that these fibrous scaffolds have a multilayered design and are made of chitin. ¹³C solid-state NMR spectroscopy, NEXAFS, and IR spectroscopy as well as chitinase digestion and test were applied in order to unequivocally prove the existence of α-chitin in all investigated species.

Original language	English
Pages (from-to)	132-140
Number of pages	9
Journal	International Journal of Biological Macromolecules
Volume	47
Issue number	2
DOIs	https://doi.org/10.1016/j.ijbiomac.2010.05.007
State	Published - Aug 2010

Keywords

Chitin
Scaffold
Sponges

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10.1016/j.ijbiomac.2010.05.007

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Ehrlich, H., Ilan, M., Maldonado, M., Muricy, G., Bavestrello, G., Kljajic, Z., Carballo, J. L., Schiaparelli, S., Ereskovsky, A., Schupp, P., Born, R., Worch, H., Bazhenov, V. V., Kurek, D., Varlamov, V., Vyalikh, D., Kummer, K., Sivkov, V., Molodtsov, S. L., ... Brunner, E. (2010). Three-dimensional chitin-based scaffolds from Verongida sponges (Demospongiae: Porifera). Part I. Isolation and identification of chitin. International Journal of Biological Macromolecules, 47(2), 132-140. https://doi.org/10.1016/j.ijbiomac.2010.05.007

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abstract = "Marine invertebrate organisms including sponges (Porifera) not only provide an abundant source of biologically active secondary metabolites but also inspire investigations to develop biomimetic composites, scaffolds and templates for practical use in materials science, biomedicine and tissue engineering. Here, we presented a detailed study of the structural and physico-chemical properties of three-dimensional skeletal scaffolds of the marine sponges Aiolochroia crassa, Aplysina aerophoba, A. cauliformis, A. cavernicola, and A. fulva (Verongida: Demospongiae). We show that these fibrous scaffolds have a multilayered design and are made of chitin. 13C solid-state NMR spectroscopy, NEXAFS, and IR spectroscopy as well as chitinase digestion and test were applied in order to unequivocally prove the existence of α-chitin in all investigated species.",

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author = "H. Ehrlich and M. Ilan and M. Maldonado and G. Muricy and G. Bavestrello and Z. Kljajic and Carballo, {J. L.} and S. Schiaparelli and A. Ereskovsky and P. Schupp and R. Born and H. Worch and Bazhenov, {V. V.} and D. Kurek and V. Varlamov and D. Vyalikh and K. Kummer and V. Sivkov and Molodtsov, {S. L.} and H. Meissner and G. Richter and E. Steck and W. Richter and S. Hunoldt and M. Kammer and S. Paasch and V. Krasokhin and G. Patzke and E. Brunner",

year = "2010",

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doi = "10.1016/j.ijbiomac.2010.05.007",

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journal = "International Journal of Biological Macromolecules",

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Ehrlich, H, Ilan, M, Maldonado, M, Muricy, G, Bavestrello, G, Kljajic, Z, Carballo, JL, Schiaparelli, S, Ereskovsky, A, Schupp, P, Born, R, Worch, H, Bazhenov, VV, Kurek, D, Varlamov, V, Vyalikh, D, Kummer, K, Sivkov, V, Molodtsov, SL, Meissner, H, Richter, G, Steck, E, Richter, W, Hunoldt, S, Kammer, M, Paasch, S, Krasokhin, V, Patzke, G & Brunner, E 2010, 'Three-dimensional chitin-based scaffolds from Verongida sponges (Demospongiae: Porifera). Part I. Isolation and identification of chitin', International Journal of Biological Macromolecules, vol. 47, no. 2, pp. 132-140. https://doi.org/10.1016/j.ijbiomac.2010.05.007

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AU - Ehrlich, H.

AU - Ilan, M.

AU - Maldonado, M.

AU - Muricy, G.

AU - Bavestrello, G.

AU - Kljajic, Z.

AU - Carballo, J. L.

AU - Schiaparelli, S.

AU - Ereskovsky, A.

AU - Schupp, P.

AU - Born, R.

AU - Worch, H.

AU - Bazhenov, V. V.

AU - Kurek, D.

AU - Varlamov, V.

AU - Vyalikh, D.

AU - Kummer, K.

AU - Sivkov, V.

AU - Molodtsov, S. L.

AU - Meissner, H.

AU - Richter, G.

AU - Steck, E.

AU - Richter, W.

AU - Hunoldt, S.

AU - Kammer, M.

AU - Paasch, S.

AU - Krasokhin, V.

AU - Patzke, G.

AU - Brunner, E.

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AB - Marine invertebrate organisms including sponges (Porifera) not only provide an abundant source of biologically active secondary metabolites but also inspire investigations to develop biomimetic composites, scaffolds and templates for practical use in materials science, biomedicine and tissue engineering. Here, we presented a detailed study of the structural and physico-chemical properties of three-dimensional skeletal scaffolds of the marine sponges Aiolochroia crassa, Aplysina aerophoba, A. cauliformis, A. cavernicola, and A. fulva (Verongida: Demospongiae). We show that these fibrous scaffolds have a multilayered design and are made of chitin. 13C solid-state NMR spectroscopy, NEXAFS, and IR spectroscopy as well as chitinase digestion and test were applied in order to unequivocally prove the existence of α-chitin in all investigated species.

KW - Chitin

KW - Scaffold

KW - Sponges

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AN - SCOPUS:77954142816

SN - 0141-8130

VL - 47

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EP - 140

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

IS - 2

ER -

Three-dimensional chitin-based scaffolds from Verongida sponges (Demospongiae: Porifera). Part I. Isolation and identification of chitin

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Keywords

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