Three-dimensional characterization of drug-encapsulating particles using STEM detector in FEG-SEM

Zahava Barkay; Ilia Rivkin; Rimona Margalit

doi:10.1016/j.micron.2008.12.003

Three-dimensional characterization of drug-encapsulating particles using STEM detector in FEG-SEM

Zahava Barkay^*, Ilia Rivkin, Rimona Margalit

^*Corresponding author for this work

Biochemistry Molecular Biology

Research output: Contribution to journal › Article › peer-review

Abstract

New drug-encapsulating particles were investigated using bright field (BF) scanning transmission electron microscopy (STEM) in a field emission gun (FEG) scanning electron microscope (SEM). Thickness characterization was done based on measuring the effective cross-section for interaction in our sample-detector configuration using calibration particles. A simplified analytical model, taking account of BF-STEM contrast and effective cross-section for interaction, was utilized for transforming projected two-dimensional BF-STEM images into three-dimensional thickness images. The three-dimensional characterization is demonstrated on a new family of biological materials composed of submicron to micron drug-free and drug-encapsulating particles. The importance of using BF-STEM in SEM, relative to other electron microscopy methods, is discussed as well as the lateral and depth resolution.

Original language	English
Pages (from-to)	480-485
Number of pages	6
Journal	Micron
Volume	40
Issue number	4
DOIs	https://doi.org/10.1016/j.micron.2008.12.003
State	Published - Jun 2009

Keywords

Drug-encapsulating particles
Low energy STEM
Mass-thickness contrast
SEM

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10.1016/j.micron.2008.12.003

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title = "Three-dimensional characterization of drug-encapsulating particles using STEM detector in FEG-SEM",

abstract = "New drug-encapsulating particles were investigated using bright field (BF) scanning transmission electron microscopy (STEM) in a field emission gun (FEG) scanning electron microscope (SEM). Thickness characterization was done based on measuring the effective cross-section for interaction in our sample-detector configuration using calibration particles. A simplified analytical model, taking account of BF-STEM contrast and effective cross-section for interaction, was utilized for transforming projected two-dimensional BF-STEM images into three-dimensional thickness images. The three-dimensional characterization is demonstrated on a new family of biological materials composed of submicron to micron drug-free and drug-encapsulating particles. The importance of using BF-STEM in SEM, relative to other electron microscopy methods, is discussed as well as the lateral and depth resolution.",

keywords = "Drug-encapsulating particles, Low energy STEM, Mass-thickness contrast, SEM",

author = "Zahava Barkay and Ilia Rivkin and Rimona Margalit",

year = "2009",

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doi = "10.1016/j.micron.2008.12.003",

language = "אנגלית",

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T1 - Three-dimensional characterization of drug-encapsulating particles using STEM detector in FEG-SEM

AU - Barkay, Zahava

AU - Rivkin, Ilia

AU - Margalit, Rimona

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N2 - New drug-encapsulating particles were investigated using bright field (BF) scanning transmission electron microscopy (STEM) in a field emission gun (FEG) scanning electron microscope (SEM). Thickness characterization was done based on measuring the effective cross-section for interaction in our sample-detector configuration using calibration particles. A simplified analytical model, taking account of BF-STEM contrast and effective cross-section for interaction, was utilized for transforming projected two-dimensional BF-STEM images into three-dimensional thickness images. The three-dimensional characterization is demonstrated on a new family of biological materials composed of submicron to micron drug-free and drug-encapsulating particles. The importance of using BF-STEM in SEM, relative to other electron microscopy methods, is discussed as well as the lateral and depth resolution.

AB - New drug-encapsulating particles were investigated using bright field (BF) scanning transmission electron microscopy (STEM) in a field emission gun (FEG) scanning electron microscope (SEM). Thickness characterization was done based on measuring the effective cross-section for interaction in our sample-detector configuration using calibration particles. A simplified analytical model, taking account of BF-STEM contrast and effective cross-section for interaction, was utilized for transforming projected two-dimensional BF-STEM images into three-dimensional thickness images. The three-dimensional characterization is demonstrated on a new family of biological materials composed of submicron to micron drug-free and drug-encapsulating particles. The importance of using BF-STEM in SEM, relative to other electron microscopy methods, is discussed as well as the lateral and depth resolution.

KW - Drug-encapsulating particles

KW - Low energy STEM

KW - Mass-thickness contrast

KW - SEM

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DO - 10.1016/j.micron.2008.12.003

M3 - מאמר

AN - SCOPUS:63549133988

VL - 40

SP - 480

EP - 485

JO - Micron

JF - Micron

SN - 0968-4328

IS - 4

ER -

Three-dimensional characterization of drug-encapsulating particles using STEM detector in FEG-SEM

Abstract

Keywords

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