Three dimension optical imaging in a high throughput layered expression system

Reut Cohen*, Gallya Gannot, Moshe Ban David, Nir Sochen, Israel Gannot

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Layered peptide array (LPA) system enables multiplex screening of biomarkers [1-3]. One of the main problems of the LPA system is the screening of the layered-membranes stack. Nowadays, each membrane is imaged separately using conventional fluorescent imaging. This process is time consuming and requires extensive manual interaction. This paper describes a general solution for optical imaging of a layered grid medium using photogrammetric methods. The suggested method enables visualization of the LPA membranes stack by using only two images of the stack. This study is a proof of concept of the suggested solution using MATLAB simulation and phantom experiments.

Original languageEnglish
Pages (from-to)19822-19835
Number of pages14
JournalOptics Express
Issue number21
StatePublished - 10 Oct 2011


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