Three cellulosomal xylanase genes in Clostridium thermocellum are regulated by both vegetative SigA (σA) and alternative SigI6 (σI6) factors

Andy Sand, Evert K. Holwerda, Natalie M. Ruppertsberger, Marybeth Maloney, Daniel G. Olson, Yakir Nataf, Ilya Borovok, Abraham L. Sonenshein, Edward A. Bayer, Raphael Lamed, Lee R. Lynd, Yuval Shoham

Research output: Contribution to journalArticlepeer-review

Abstract

Clostridium thermocellum efficiently degrades crystalline cellulose by a high molecular weight protein complex, the cellulosome. The bacterium regulates its cellulosomal genes using a unique extracellular biomass-sensing mechanism that involves alternative sigma factors and extracellular carbohydrate-binding modules attached to intracellular anti-sigma domains. In this study, we identified three cellulosomal xylanase genes that are regulated by the σI6/RsgI6 system by utilizing sigI6 and rsgI6 knockout mutants together with primer extension analysis. Our results indicate that cellulosomal genes are expressed from both alternative σI6 and σA vegetative promoters.

Original languageEnglish
Article number37336
Pages (from-to)3133-3140
Number of pages8
JournalFEBS Letters
Volume589
Issue number20
DOIs
StatePublished - 7 Oct 2015

Keywords

  • Alternative sigma factor
  • Alternative sigma factor
  • Biomass sensing
  • Cellulosome
  • Clostridium thermocellum
  • Regulation
  • Xylanase

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