@article{e8715d5d3c844129aaf850af353d5c1f,
title = "Three cellulosomal xylanase genes in Clostridium thermocellum are regulated by both vegetative SigA (σA) and alternative SigI6 (σI6) factors",
abstract = "Clostridium thermocellum efficiently degrades crystalline cellulose by a high molecular weight protein complex, the cellulosome. The bacterium regulates its cellulosomal genes using a unique extracellular biomass-sensing mechanism that involves alternative sigma factors and extracellular carbohydrate-binding modules attached to intracellular anti-sigma domains. In this study, we identified three cellulosomal xylanase genes that are regulated by the σI6/RsgI6 system by utilizing sigI6 and rsgI6 knockout mutants together with primer extension analysis. Our results indicate that cellulosomal genes are expressed from both alternative σI6 and σA vegetative promoters.",
keywords = "Alternative sigma factor, Alternative sigma factor, Biomass sensing, Cellulosome, Clostridium thermocellum, Regulation, Xylanase",
author = "Andy Sand and Holwerda, \{Evert K.\} and Ruppertsberger, \{Natalie M.\} and Marybeth Maloney and Olson, \{Daniel G.\} and Yakir Nataf and Ilya Borovok and Sonenshein, \{Abraham L.\} and Bayer, \{Edward A.\} and Raphael Lamed and Lynd, \{Lee R.\} and Yuval Shoham",
note = "Publisher Copyright: {\textcopyright} 2015 Federation of European Biochemical Societies.",
year = "2015",
month = oct,
day = "7",
doi = "10.1016/j.febslet.2015.08.026",
language = "אנגלית",
volume = "589",
pages = "3133--3140",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "John Wiley and Sons Inc.",
number = "20",
}