Three cellulosomal xylanase genes in Clostridium thermocellum are regulated by both vegetative SigA (σA) and alternative SigI6 (σI6) factors

Andy Sand; Evert K. Holwerda; Natalie M. Ruppertsberger; Marybeth Maloney; Daniel G. Olson; Yakir Nataf; Ilya Borovok; Abraham L. Sonenshein; Edward A. Bayer; Raphael Lamed; Lee R. Lynd; Yuval Shoham

doi:10.1016/j.febslet.2015.08.026

Three cellulosomal xylanase genes in Clostridium thermocellum are regulated by both vegetative SigA (σ^A) and alternative SigI6 (σ^I6) factors

Andy Sand, Evert K. Holwerda, Natalie M. Ruppertsberger, Marybeth Maloney, Daniel G. Olson, Yakir Nataf, Ilya Borovok, Abraham L. Sonenshein, Edward A. Bayer, Raphael Lamed, Lee R. Lynd, Yuval Shoham^*

^*Corresponding author for this work

Biotechnology

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Clostridium thermocellum efficiently degrades crystalline cellulose by a high molecular weight protein complex, the cellulosome. The bacterium regulates its cellulosomal genes using a unique extracellular biomass-sensing mechanism that involves alternative sigma factors and extracellular carbohydrate-binding modules attached to intracellular anti-sigma domains. In this study, we identified three cellulosomal xylanase genes that are regulated by the σ^I6/RsgI6 system by utilizing sigI6 and rsgI6 knockout mutants together with primer extension analysis. Our results indicate that cellulosomal genes are expressed from both alternative σ^I6 and σ^A vegetative promoters.

Original language	English
Article number	37336
Pages (from-to)	3133-3140
Number of pages	8
Journal	FEBS Letters
Volume	589
Issue number	20
DOIs	https://doi.org/10.1016/j.febslet.2015.08.026
State	Published - 7 Oct 2015

Funding

Funders	Funder number
BioEnergy Science Center
Israeli Center of Research Excellence
Ministry of Environmental Protection, Israel
Sidney E. Frank Foundation
U.S. Department of Energy Bioenergy Research Center
Office of Science
Biological and Environmental Research
Leona M. and Harry B. Helmsley Charitable Trust
Nancy and Stephen Grand Technion Energy Program
Weizmann Institute of Science
United States-Israel Binational Science Foundation	2011049
Israel Science Foundation	715/12, 500/10, 1349/13, 24/11
Israeli Centers for Research Excellence	152/11
Technion-Israel Institute of Technology

Keywords

Alternative sigma factor
Alternative sigma factor
Biomass sensing
Cellulosome
Clostridium thermocellum
Regulation
Xylanase

Access to Document

10.1016/j.febslet.2015.08.026

Cite this

Sand, A., Holwerda, E. K., Ruppertsberger, N. M., Maloney, M., Olson, D. G., Nataf, Y., Borovok, I., Sonenshein, A. L., Bayer, E. A., Lamed, R., Lynd, L. R., & Shoham, Y. (2015). Three cellulosomal xylanase genes in Clostridium thermocellum are regulated by both vegetative SigA (σ^A) and alternative SigI6 (σ^I6) factors. FEBS Letters, 589(20), 3133-3140. Article 37336. https://doi.org/10.1016/j.febslet.2015.08.026

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title = "Three cellulosomal xylanase genes in Clostridium thermocellum are regulated by both vegetative SigA (σA) and alternative SigI6 (σI6) factors",

abstract = "Clostridium thermocellum efficiently degrades crystalline cellulose by a high molecular weight protein complex, the cellulosome. The bacterium regulates its cellulosomal genes using a unique extracellular biomass-sensing mechanism that involves alternative sigma factors and extracellular carbohydrate-binding modules attached to intracellular anti-sigma domains. In this study, we identified three cellulosomal xylanase genes that are regulated by the σI6/RsgI6 system by utilizing sigI6 and rsgI6 knockout mutants together with primer extension analysis. Our results indicate that cellulosomal genes are expressed from both alternative σI6 and σA vegetative promoters.",

keywords = "Alternative sigma factor, Alternative sigma factor, Biomass sensing, Cellulosome, Clostridium thermocellum, Regulation, Xylanase",

author = "Andy Sand and Holwerda, \{Evert K.\} and Ruppertsberger, \{Natalie M.\} and Marybeth Maloney and Olson, \{Daniel G.\} and Yakir Nataf and Ilya Borovok and Sonenshein, \{Abraham L.\} and Bayer, \{Edward A.\} and Raphael Lamed and Lynd, \{Lee R.\} and Yuval Shoham",

note = "Publisher Copyright: {\textcopyright} 2015 Federation of European Biochemical Societies.",

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doi = "10.1016/j.febslet.2015.08.026",

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Sand, A, Holwerda, EK, Ruppertsberger, NM, Maloney, M, Olson, DG, Nataf, Y, Borovok, I, Sonenshein, AL, Bayer, EA, Lamed, R, Lynd, LR & Shoham, Y 2015, 'Three cellulosomal xylanase genes in Clostridium thermocellum are regulated by both vegetative SigA (σ^A) and alternative SigI6 (σ^I6) factors', FEBS Letters, vol. 589, no. 20, 37336, pp. 3133-3140. https://doi.org/10.1016/j.febslet.2015.08.026

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AU - Sand, Andy

AU - Holwerda, Evert K.

AU - Ruppertsberger, Natalie M.

AU - Maloney, Marybeth

AU - Olson, Daniel G.

AU - Nataf, Yakir

AU - Borovok, Ilya

AU - Sonenshein, Abraham L.

AU - Bayer, Edward A.

AU - Lamed, Raphael

AU - Lynd, Lee R.

AU - Shoham, Yuval

PY - 2015/10/7

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N2 - Clostridium thermocellum efficiently degrades crystalline cellulose by a high molecular weight protein complex, the cellulosome. The bacterium regulates its cellulosomal genes using a unique extracellular biomass-sensing mechanism that involves alternative sigma factors and extracellular carbohydrate-binding modules attached to intracellular anti-sigma domains. In this study, we identified three cellulosomal xylanase genes that are regulated by the σI6/RsgI6 system by utilizing sigI6 and rsgI6 knockout mutants together with primer extension analysis. Our results indicate that cellulosomal genes are expressed from both alternative σI6 and σA vegetative promoters.

AB - Clostridium thermocellum efficiently degrades crystalline cellulose by a high molecular weight protein complex, the cellulosome. The bacterium regulates its cellulosomal genes using a unique extracellular biomass-sensing mechanism that involves alternative sigma factors and extracellular carbohydrate-binding modules attached to intracellular anti-sigma domains. In this study, we identified three cellulosomal xylanase genes that are regulated by the σI6/RsgI6 system by utilizing sigI6 and rsgI6 knockout mutants together with primer extension analysis. Our results indicate that cellulosomal genes are expressed from both alternative σI6 and σA vegetative promoters.

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KW - Biomass sensing

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KW - Clostridium thermocellum

KW - Regulation

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