Three cellulosomal xylanase genes in Clostridium thermocellum are regulated by both vegetative SigA (σA) and alternative SigI6 (σI6) factors

Andy Sand; Evert K. Holwerda; Natalie M. Ruppertsberger; Marybeth Maloney; Daniel G. Olson; Yakir Nataf; Ilya Borovok; Abraham L. Sonenshein; Edward A. Bayer; Raphael Lamed; Lee R. Lynd; Yuval Shoham

doi:10.1016/j.febslet.2015.08.026

Three cellulosomal xylanase genes in Clostridium thermocellum are regulated by both vegetative SigA (σ^A) and alternative SigI6 (σ^I6) factors

Andy Sand, Evert K. Holwerda, Natalie M. Ruppertsberger, Marybeth Maloney, Daniel G. Olson, Yakir Nataf, Ilya Borovok, Abraham L. Sonenshein, Edward A. Bayer, Raphael Lamed, Lee R. Lynd, Yuval Shoham

Biotechnology

Research output: Contribution to journal › Article › peer-review

Abstract

Clostridium thermocellum efficiently degrades crystalline cellulose by a high molecular weight protein complex, the cellulosome. The bacterium regulates its cellulosomal genes using a unique extracellular biomass-sensing mechanism that involves alternative sigma factors and extracellular carbohydrate-binding modules attached to intracellular anti-sigma domains. In this study, we identified three cellulosomal xylanase genes that are regulated by the σ^I6/RsgI6 system by utilizing sigI6 and rsgI6 knockout mutants together with primer extension analysis. Our results indicate that cellulosomal genes are expressed from both alternative σ^I6 and σ^A vegetative promoters.

Original language	English
Article number	37336
Pages (from-to)	3133-3140
Number of pages	8
Journal	FEBS Letters
Volume	589
Issue number	20
DOIs	https://doi.org/10.1016/j.febslet.2015.08.026
State	Published - 7 Oct 2015

Keywords

Alternative sigma factor
Alternative sigma factor
Biomass sensing
Cellulosome
Clostridium thermocellum
Regulation
Xylanase

Access to Document

10.1016/j.febslet.2015.08.026

Cite this

Sand, A., Holwerda, E. K., Ruppertsberger, N. M., Maloney, M., Olson, D. G., Nataf, Y., Borovok, I., Sonenshein, A. L., Bayer, E. A., Lamed, R., Lynd, L. R., & Shoham, Y. (2015). Three cellulosomal xylanase genes in Clostridium thermocellum are regulated by both vegetative SigA (σ^A) and alternative SigI6 (σ^I6) factors. FEBS Letters, 589(20), 3133-3140. [37336]. https://doi.org/10.1016/j.febslet.2015.08.026

@article{e8715d5d3c844129aaf850af353d5c1f,

title = "Three cellulosomal xylanase genes in Clostridium thermocellum are regulated by both vegetative SigA (σA) and alternative SigI6 (σI6) factors",

abstract = "Clostridium thermocellum efficiently degrades crystalline cellulose by a high molecular weight protein complex, the cellulosome. The bacterium regulates its cellulosomal genes using a unique extracellular biomass-sensing mechanism that involves alternative sigma factors and extracellular carbohydrate-binding modules attached to intracellular anti-sigma domains. In this study, we identified three cellulosomal xylanase genes that are regulated by the σI6/RsgI6 system by utilizing sigI6 and rsgI6 knockout mutants together with primer extension analysis. Our results indicate that cellulosomal genes are expressed from both alternative σI6 and σA vegetative promoters.",

keywords = "Alternative sigma factor, Alternative sigma factor, Biomass sensing, Cellulosome, Clostridium thermocellum, Regulation, Xylanase",

author = "Andy Sand and Holwerda, {Evert K.} and Ruppertsberger, {Natalie M.} and Marybeth Maloney and Olson, {Daniel G.} and Yakir Nataf and Ilya Borovok and Sonenshein, {Abraham L.} and Bayer, {Edward A.} and Raphael Lamed and Lynd, {Lee R.} and Yuval Shoham",

note = "Publisher Copyright: {\textcopyright} 2015 Federation of European Biochemical Societies.",

year = "2015",

month = oct,

day = "7",

doi = "10.1016/j.febslet.2015.08.026",

language = "אנגלית",

volume = "589",

pages = "3133--3140",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "20",

}

Sand, A, Holwerda, EK, Ruppertsberger, NM, Maloney, M, Olson, DG, Nataf, Y, Borovok, I, Sonenshein, AL, Bayer, EA, Lamed, R, Lynd, LR & Shoham, Y 2015, 'Three cellulosomal xylanase genes in Clostridium thermocellum are regulated by both vegetative SigA (σ^A) and alternative SigI6 (σ^I6) factors', FEBS Letters, vol. 589, no. 20, 37336, pp. 3133-3140. https://doi.org/10.1016/j.febslet.2015.08.026

TY - JOUR

T1 - Three cellulosomal xylanase genes in Clostridium thermocellum are regulated by both vegetative SigA (σA) and alternative SigI6 (σI6) factors

AU - Sand, Andy

AU - Holwerda, Evert K.

AU - Ruppertsberger, Natalie M.

AU - Maloney, Marybeth

AU - Olson, Daniel G.

AU - Nataf, Yakir

AU - Borovok, Ilya

AU - Sonenshein, Abraham L.

AU - Bayer, Edward A.

AU - Lamed, Raphael

AU - Lynd, Lee R.

AU - Shoham, Yuval

PY - 2015/10/7

Y1 - 2015/10/7

N2 - Clostridium thermocellum efficiently degrades crystalline cellulose by a high molecular weight protein complex, the cellulosome. The bacterium regulates its cellulosomal genes using a unique extracellular biomass-sensing mechanism that involves alternative sigma factors and extracellular carbohydrate-binding modules attached to intracellular anti-sigma domains. In this study, we identified three cellulosomal xylanase genes that are regulated by the σI6/RsgI6 system by utilizing sigI6 and rsgI6 knockout mutants together with primer extension analysis. Our results indicate that cellulosomal genes are expressed from both alternative σI6 and σA vegetative promoters.

AB - Clostridium thermocellum efficiently degrades crystalline cellulose by a high molecular weight protein complex, the cellulosome. The bacterium regulates its cellulosomal genes using a unique extracellular biomass-sensing mechanism that involves alternative sigma factors and extracellular carbohydrate-binding modules attached to intracellular anti-sigma domains. In this study, we identified three cellulosomal xylanase genes that are regulated by the σI6/RsgI6 system by utilizing sigI6 and rsgI6 knockout mutants together with primer extension analysis. Our results indicate that cellulosomal genes are expressed from both alternative σI6 and σA vegetative promoters.

KW - Alternative sigma factor

KW - Biomass sensing

KW - Cellulosome

KW - Clostridium thermocellum

KW - Regulation

KW - Xylanase

UR - http://www.scopus.com/inward/record.url?scp=84943201477&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2015.08.026

DO - 10.1016/j.febslet.2015.08.026

M3 - מאמר

C2 - 26320414

AN - SCOPUS:84943201477

VL - 589

SP - 3133

EP - 3140

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 20

M1 - 37336

ER -

Three cellulosomal xylanase genes in Clostridium thermocellum are regulated by both vegetative SigA (σ^A) and alternative SigI6 (σ^I6) factors

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this